玛咖结合运动疗法对糖尿病大鼠肾线粒体呼吸链酶活性及肾功能的影响  

Effects of Maca combined exercise on activity of renal mitochondrial respiratory chain enzymes and renal function in diabetic rats

作　　者：彭丽娜[1] 宁尚斌[1] 王光光[1] 常世民[1] 张玉峰[1] 李艳霞[1] PENG Lina;NING Shangbin;WANG Guangguang;CHANG Shimin;ZHANG Yufeng;LI Yanxia(Department of Human Movement Science,Langfang Normal University,Langfang 065000,China)

机构地区：[1]廊坊师范学院运动人体科学教研室,河北廊坊065000

出　　处：《中国比较医学杂志》2019年第1期64-68,共5页Chinese Journal of Comparative Medicine

基　　金：2016年廊坊师范学院自然科学研究项目(LSLY201605)

摘　　要：目的观察玛咖结合运动疗法对糖尿病大鼠肾线粒体呼吸酶活性及肾功能的影响。方法将40只糖尿病模型大鼠随机分为模型对照组、玛咖组、运动组和玛咖+运动组。玛咖组和玛咖+运动组每日灌胃玛咖粉剂(5 g/kg),运动组和玛咖+运动组进行跑台训练。6周后各组大鼠进行力竭运动(35 m/min),随后即刻麻醉提取肾线粒体,测定线粒体呼吸链酶RCCI、RCCII、RCCIII、RCCIV活性及血尿素氮(blood urea nitrogen,BUN)、血清肌酐(serum creatinine,SCr)、血糖(blood glucose)含量。结果与对照组比较,玛咖组大鼠肾线粒体呼吸链酶RCCII,运动组RCCI、RCCII,玛咖+运动组RCCI、RCCII、RCCIV活性显著升高;与玛咖组比较,玛咖+运动组RCCI、RCCII活性显著升高;与运动组比较,玛咖+运动组RCCII活性显著升高。与对照组比较,玛咖组、玛咖+运动组大鼠血尿素氮含量,运动组、玛咖+运动组血清肌酐、血糖水平降低,差异有显著性(P<0. 05或P<0. 01);与玛咖组比较,玛咖+运动组血清肌酐、血糖水平降低,差异有显著性(P<0. 05);与运动组比较,玛咖+运动组血尿素氮、血糖水平降低,差异有显著性(P<0. 05)。结论玛咖结合运动疗法可以提高老龄糖尿病大鼠肾线粒呼吸功能,提高机体氧化磷酸化水平,改善肾机能。Objective To observe the effect of Maca combined exercise therapy on mitochondrial respiratory enzyme activity and renal function in diabetic rats.Methods Forty diabetic rats were randomly divided into model control group,Maca group,training group and Maca+training group.The training and Maca+training groups underwent treadmill exercise training every day(5 g/kg).After 6 weeks,rats in each group were subjected to exhaustive exercise(35 m/min)followed by immediate extraction of renal mitochondria under anesthesia.Activity of the mitochondrial respiratory chain enzymes RCCI,RCCII,RCCIII and RCCIV,blood urea nitrogen(BUN),serum creatinine(SCr),and blood glucose contents were measured.Results Compared with the control group,the Maca group RCCII,training group RCCI and RCCII,and Maca+training group RCCI,RCCII and RCCIV activities were significantly increased.Compared with the Maca group,RCCI and RCCII activities in the Maca+training group were significantly increased.Compared with the training group,the RCCII activity in the Maca+training group was significantly increased.Compared with the control group,the content of blood urea nitrogen in the Maca and Maca+training groups,and the levels of serum creatinine and blood glucose in the training group and Maca+training group were significantly decreased(P<0.05 or P<0.01).Compared with the Maca group,the levels of serum creatinine and blood glucose were significantly decreased in the Maca+training group(P<0.05).Compared with the training group,the levels of blood urea nitrogen and blood glucose in the Maca+training group were significantly decreased(P<0.05).Conclusions Maca combined with exercise therapy improved the respiratory function,increased the level of oxidative phosphorylation,and improved kidney function in aged diabetic rats.

关 键 词：玛咖 运动 糖尿病大鼠 肾线粒体 呼吸酶 肾功能 

分 类 号：R-33[医药卫生]