肝癌患者TACE治疗前后外周血PD-1、PD-L1的表达及临床意义  

Expression and clinical significance of PD-1/PD-L1 in hepatocellular carcinoma patients

作　　者：程小珍[1] 李晓东 王顺兰[3] 谢宗宙[1] 毛山山[1] 朱燕兴 崔荣花[1] 王美清[1] Xiao-zhen Cheng;Xiao-dong Li;Shun-lan Wang;Zong-zhou Xie;Shan-shan Mao;Yan-xing Zhu;Rong-hua Cui;Mei-qing Wang(Department of Tumor-Chemotherapy,Central South University Xiangya School of Medicine Affiliated Haikou Hospital,Haikou,Hainan 570208,China;Clinical Immunology Laboratory,Davis School of Medicine,University of California,California,CA95817,USA;Central laboratory,Central South University Xiangya School of Medicine Affiliated Haikou Hospital,Haikou,Hainan 570208,China)

机构地区：[1]中南大学湘雅医学院附属海口医院肿瘤化疗科,海南海口570208 [2]加州大学戴维斯分校-医学院临床免疫研究室,美国加州ca95817 [3]中南大学湘雅医学院附属海口医院中心实验室,海南海口570208

出　　处：《中国现代医学杂志》2019年第3期33-37,共5页China Journal of Modern Medicine

基　　金：海南省海口市重点科技计划项目(No:2014-084)

摘　　要：目的探讨原发性肝癌患者行肝动脉化疗栓塞术(TACE)前后外周血程序性死亡分子1(PD-1)、配体(PD-L1)的表达及临床意义。方法采集中南大学湘雅医学院附属海口医院28例中晚期肝癌患者(肝癌组)TACE治疗前后外周血及20例健康体检者(对照组)外周血标本,流式细胞仪检测CD4+T、CD8+T细胞及抗原提呈细胞(APC)表面PD-1、PD-L1的表达,并随访PD-1、PD-L1表达与肿瘤分化、疗效的关系。结果肝癌组外周血CD4^+T、CD8^+T细胞及APC表面PD-1、PD-L1表达与对照组比较,差异有统计学意义(P <0.05),肝癌组均高于对照组;治疗前后肝癌组外周血CD4^+T、CD8^+T及APC表面PD-L1表达,差异有统计学意义(P <0.05),治疗后较治疗前下降;CD4+T、CD8^+T及APC表面PD-1表达也较治疗前下降,但差异无统计学意义(P>0.05);细胞分化差的肝癌组患者外周血PD-1、PD-L1表达较高;治疗效果差的肝癌组外周血PD-1、PD-L1表达较高。结论肝癌患者外周血PD-1、PD-L1表达升高,治疗前后监测PD-1、PD-L1表达可能预测患者预后状态。Objective To investigate the expression of programmed death 1(PD-1)/programmed death ligand 1(PD-L1)in peripheral blood of patients with primary hepatocellular carcinoma(HCC)pre and post-transcatheter arterial chemoembolization(TACE)and its clinical significance.Methods A total of 20 healthy volunteers and 28 HCC patients were enrolled.The levels of PD-1 and PD-L1 on the surface of CD4^+T cells,CD8^+T cells and antigenpresenting cells(APC)in peripheral blood were detected by flow cytometry.Relationship between the expression of PD-1 and PD-L1 and the differentiation and outcomes of tumor was analyzed.Results The levels of PD-1 and PD-L1 on the surface of CD4^+ T cells,CD8^+ T cells and APC in peripheral blood of HCC patients were increased than those in healthy volunteers(P<0.05).The expression of PD-L1 on the surface of CD4^+ T cells,CD8^+ T cells and APC in peripheral blood of HCC patients was decreased after TACE comparing to those in patients prior to treatments,while no obvious difference was identified in patients prior to and post treatments.High expression of PD-1 and PD-L1 was determined in patients with poor differentiation or poor outcomes.Conclusions The levels of PD-1 and PD-L1 in peripheral blood of patients with hepatocellular carcinoma are responsive to TACE and clinical prognosis.

关 键 词：肝肿瘤 化学栓塞 治疗性 程序性死亡分子1 程序性死亡分子配体1 

分 类 号：R735.7[医药卫生—肿瘤]