碱提法提取地鳖虫多糖正交试验研究  

Orthogonal Test on Extraction of Polysaccharide Eupolyphaga sinensis by Alkali Extraction

作　　者：罗志文[1,2] 杨舒涵 张跃华[1] 缪天琳[1] 周清波[1] 赵永勋[1] Luo Zhiwen;Yang Shuhan;Zhang Yuehua;Miao Tianlin;Zhou Qingbo;Zhao Yongwun(School of Life Sciences,Jiamusi University,Jiamusi,Heilongjiang 154007;Institute of applied insect,Jiamusi University)

机构地区：[1]佳木斯大学生命科学学院,黑龙江佳木斯154007 [2]佳木斯大学应用昆虫研究所,黑龙江佳木斯154007

出　　处：《中国林副特产》2019年第1期16-17,共2页Forest By-product and Speciality in China

基　　金：黑龙江省卫生厅科研项目(2012-269)研究内容

摘　　要：采用碱提法对地鳖虫多糖进行提取试验,以碱液浓度、提取温度、提取时间等因素做正交试验,用苯酚-硫酸进行多糖含量测定,最佳的提取工艺条件为最佳的提取工艺条件为A3B2C1,即碱液浓度为0.03mol/L,提取时间1h,提取温度为60℃。The extraction of Polysaccharides from Trionyx sinensis was studied by alkali extraction method.The orthogonal experiments were carried out with the factors of alkali concentration,extraction temperature and extraction time.The content of polysaccharides was determined by phenol-sulfuric acid.The optimum extraction conditions were A3B2C1,that is,the concentration of alkali solution was 0.03 mol/L,extraction time was 1 h,and extraction temperature was 60 C.

关 键 词：地鳖虫多糖 碱提法 提取工艺 正交试验 

分 类 号：X174[环境科学与工程—环境科学]