基于EST-SSR标记的珠子参野生种质资源遗传多样性分析  

Analysis of Genetic Diversity of Panax japonicus by EST-SSR Markers

作　　者：杨维泽[1] 左应梅[1] 张金渝[1] 杨天梅[1] 杨绍兵[1] 杨美权[1] 邓先能[1] 许宗亮[1] 陈秀花 李新华 YANG Wei-ze;ZUO Ying-mei;ZHANG Jin-yu;YANG Tian-mei;YANG Shao-bing;YANG Mei-quan;DENG Xian-neng;XU Zong-liang;CHEN Xiu-hua;LI Xin-hua(Institute of Medicinal Plant, Yunnan Academy of Agricultural Sciences, Yunnan Kunming 650231,China;Gongshan Bureau of Agriculture, Yunnan Gongshan 673500,China)

机构地区：[1]云南省农业科学院药用植物研究所,云南昆明650223 [2]贡山县农业局,云南贡山673500

出　　处：《西南农业学报》2019年第1期49-54,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金(81260610);国家科技支撑计划项目(2011BAI13B01-02);云南省科技厅项目(2011CG024)

摘　　要：【目的】利用EST-SSR标记分析云南省珠子参野生资源的遗传多样性。【方法】利用17对人参属EST-SSR引物对云南省珠子参主要分布区的19个珠子参野生居群进行遗传多样性分析。【结果】在物种水平,17对人参属EST-SSR引物在19个珠子参居群中共检测到346个位点,其中多态位点296个,平均为17. 4个,珠子参的等位基因数(A)为1. 8555,Nei’s基因多样性(H)为0. 2023、Shannon指数(I)为0. 3230;在居群水平,19个居群的等位基因数(A)为1. 0954～1. 2977,Nei’s基因多样性(H)为0. 1043～0. 1387,Shannon指数(I)为0. 0309～0. 1077,多态位点数(PPB)为8. 67%～29. 77%。居群间的遗传分化系数(Gst)为0. 6357,居群每代迁移数(Nm)为0. 2866。遗传相似度和聚类分析的结果表明,19份珠子参居群被划分为2个大类群,其中第二类群16个居群被分为4个小类群。【结论】珠子参总体上具有丰富的遗传多样性,但各个居群的遗传多样性不高,居群间缺乏基因交流;居群间遗传分化水平高,遗传差异主要存在于居群间;聚类分析显示,不同表型的珠子参虽然形态差异较大,但遗传差异不大。【Objective】Genetic diversity of wild Resources P.japonicus in Yunnan province by EST-SSR markers were analyzed.【Method】The genetic diversity and genetic structure of 19 P.japonicas populations in the main distribution area of Yunnan province were analyzed by using 17 pairs of EST-SSR molecular markers.【Result】In species level,a total of 346 mutation sites were detected in 19 populations of by 17 EST-SSRs primer pairs from Panax,among which 296 were polymorphic loci.The allele(A)number was 1.8555 in P.japonicus,the Nei's(H)was 0.2023,and Shannon's information index(I)was 0.3230.In population level,the allele was 1.0954-1.2977 in 19 populations,Nei's gene diversity(H)was from 0.1043 to 0.1387 and the range of Shannon's information index(I)was from 0.0309 to 0.1077.Total percentage of polymorphic loci(PPB)was from 8.67%to 29.77%in these populations.Coefficient of gene differentiation(Gst)was 0.6357 among populations of P.japonicus.And the populations transport number(Nm)of one generation was 0.2866.Genetic similarity and cluster analysis results showed that 19 populations of P.japonicus were divided into two large groups,among which 16 populations could be classified into 4 groups.【Conclusionl】P.japonicus had more genetic diversity,but had low genetic diversity in each populations and was lack of genetic communication among populations.The level of genetic differentiation among populations was higher,and the genetic difference was mainly existed among populations.Although the phenotypic diversity in different populations was great,but the genetic diversity was small.

关 键 词：珠子参 EST-SSR标记 遗传多样性 

分 类 号：R284[医药卫生—中药学]