CRISPR/Cas9技术定点编辑水稻谷蛋白基因GluA  

Targeted editing of rice gluten gene GluA3 by CRISPR/Cas9 technology

作　　者：周优 林冬枝[1,2] 董彦君[1,2,3] ZHOU You;LIN Dong-zhi;DONG Yan-jun(College of Life Sciences,Shanghai Normal University,Shanghai 200234,China;Institute of Genetics,Shanghai Normal University,Shanghai 200234,China;Shanghai Key Laboratory of Plant Molecular Sciences,Shanghai 200234,China)

机构地区：[1]上海师范大学生命科学学院,上海200234 [2]上海师范大学遗传研究所,上海200234 [3]上海市植物分子科学重点实验室,上海200234

出　　处：《上海农业学报》2019年第1期22-28,共7页Acta Agriculturae Shanghai

基　　金：国家重点研发项目(2016YFD0100902);上海市科委项目(16ZR14253000;1639190070010);上海市教委项目(2017-01-07-00-02-E00039)

摘　　要：通过CRISPR/Cas9基因编辑技术对粳稻品种‘嘉花1号’的谷蛋白基因GluA3(LOC_Os03 g31360)进行编辑获得转基因T0代植株,并对其衍生的T1代28个单株进行鉴定分析。结果表明:获得了两种类型的GluA3编辑突变植株;突变体的未成熟胚乳中GluA3 RNA表达水平明显下调;突变体谷蛋白含量呈下降趋势。对‘嘉花1号’野生型及突变体水稻农艺性状考查发现,突变体水稻穗数和产量下降明显。试验表明,CRISPR/Cas9技术可有效编辑水稻谷蛋白基因,可为水稻谷蛋白品质育种提供理论指导。The gluten gene GluA3(LOC_Os03 g31360)in japonica rice variety‘Jiahua 1’was edited by CRISPR Cas9 technology,and 28 T 1 individuals derived from the transgenic T 0 generation were identified and analyzed.The results showed that two types of GluA3 edited-mutant plants were obtained;the expression levels of GluA3 RNA in immature endosperm of the two edited-mutants were significantly down-regulated;the gluten content of the mutants had a downward trend.The agronomic traits of‘Jiahua 1’wild type and mutant rice were also examined.It was found that the panicle number and yield of mutant rice decreased obviously.The research showed that CRISPR Cas9 technology could edit gluten gene effectively and it would provide theoretical guidance for breeding and improving quality of gluten in rice.

关 键 词：水稻 CRISPRCas9 贮藏蛋白 谷蛋白基因 

分 类 号：S511.03[农业科学—作物学]