宫颈上皮脱落细胞B-myb基因表达和临床意义  

Expression and clinical significance of B-myb gene in exfoliated cervical epithelial cells

作　　者：刘秀娜 于海涛 李芳 Liu Xiuna;Yu Haitao;Li Fang(Clinical Laboratory,Weihai Central Hospital,Weihai 264400,China)

机构地区：[1]威海市中心医院检验科,264400

出　　处：《国际医药卫生导报》2019年第2期227-230,共4页International Medicine and Health Guidance News

摘　　要：目的 分析不同宫颈液基细胞学(TCT)检测和HPV检测结果的宫颈上皮脱落细胞B-myb基因表达差异,以探讨B-myb基因在宫颈癌筛查中的作用。方法 收集315例宫颈病变患者的宫颈上皮脱落细胞标本。根据TCT结果分为正常细胞组(NC组)118例,不能明确意义的非典型鳞状细胞组(ASCUS组)61例,可疑非典型鳞状病变细胞组(ASCH组)46例,低度鳞状上皮内病变组(LSIL组)43例,高度鳞状上皮内病变组(HSIL组)30例,宫颈癌组(SCC组)17例。根据HPV检测结果分为HPV16+和/或HPV18+组[A组,包含有HPV16+和/或HPV18+,且合并其他基因型HPV+的多重感染者]83例,其他基因型的HPV+组(B组)78例,HPV-组(C组)154例。分析TCT结果分组和HPV检测结果分组间的B-myb基因表达量差异。结果 TCT检测结果组间宫颈脱落细胞B-myb基因表达量差异:ASCUS组、ASCH组、LSIL组、HSIL组、SCC组均明显高于NC组(P<0.05);LSIL组、HSIL组、SCC组均明显高于ASCUS组、ASCH组(P<0.05);HSIL组、SCC组均明显高于LSIL组(P<0.05);SCC组明显高于HSIL组(P<0.05)。HPV检测结果组间宫颈脱落细胞B-myb基因表达量差异:A组、B组明显高于C组(P<0.05),A组明显高于B组(P<0.05)。结论 HPV16/18感染会引起B-myb基因表达异常,引起细胞周期调节异常而使细胞异常增殖,最终引起宫颈组织的癌变。故检测宫颈上皮脱落细胞中B-myb基因表达量改变可能是宫颈癌更有效的筛查指标。Objective To analyze the difference of B-myb gene expression in exfoliated cervical epithelial cells with different detection results by cervical liquid-based cytology(TCT)and HPV assays,and to explore the role of B-myb gene in cervical cancer screening.Methods The exfoliated cervical epithelial cells from 315 patients with cervical lesions were collected.According to TCT results,they were divided into normal cell group(NC group,118 cases),atypical squamous cell of undetermined significance(ASCUS)group(61 cases),suspected atypical squamous cell(ASCH)group(46 cases),low-grade squamous intraepithelial lesion(LSIL)group(43 cases),high-grade squamous intraepithelial lesion(HSIL)group(30 cases),cervical cancer group(SCC group,17 cases);according to the results of HPV detection,they were divided into HPV16+and/or HPV18+group(group A,including 83 cases of multiple infection with HPV16+and/or HPV18+and other genotypes of HPV+),other genotypes of HPV+group(group B,78 cases),HPV-group(group C,154 cases).The differences of B-myb gene expression among different TCT results groups and HPV test results groups were analyzed.Results The expression of B-myb gene in exfoliated cervical epithelial cells was significantly higher in ASCUS group,ASCH group,LSIL group,HSIL group,and SCC group than that in NC group(P<0.05);the expression of B-myb gene in LSIL group,HSIL group,and SCC group was significantly higher than that in ASCUS group and ASCH group(P<0.05);the expression of B-myb gene in HSIL group and SCC group was significantly higher than that in LSIL group(P<0.05);the expression of B-myb gene in SCC group was significantly higher than that in HSIL group(P<0.05).The expression level of B-myb gene in exfoliated cervical epithelial cells was significantly higher in group A and group B than that in group C(P<0.05),and the expression level of B-myb gene was significantly higher in group A than that in group B(P<0.05).Conclusion HPV16/18 infection can cause abnormal B-myb gene expression,abnormal cell cycle regulation and abnormal ce

关 键 词：宫颈上皮脱落细胞 B-myb基因 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]