miR383调控PRDX3表达及对人髓母细胞瘤增殖和凋亡影响的研究  

Regulation of PRDX3 Expression by miR383 and Its Effect on Proliferation and Apoptosis of Human Medulloblastoma

作　　者：王少增 高梅兰[2] 侯俊环 WANG Shao-zeng;GAO Mei-lan;HOU Jun-huan(Department of Clinical Laboratory,Qingyun County People′s Hospital of Shandong,Qingyun253700,China)

机构地区：[1]山东省庆云县人民医院检验科,山东庆云253700 [2]滨州医学院附属医院检验科,山东滨州256603 [3]山东省庆云县人民医院预防保健科,山东庆云253700

出　　处：《标记免疫分析与临床》2019年第1期139-142,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探讨muR383调控PRDX3表达及对人髓母细胞瘤增殖和凋亡的影响。方法新鲜组织样品来自于某医院2015年12月至2017年12月就诊的人髓母细胞瘤患者35例;另10例非肿瘤脑组织来自于该院病理科,作为对照组。人髓母瘤细胞系Daoy购自中国科学院细胞资源中心。采用Western blot法分别检测PRDX3在人髓母细胞瘤组织、Daoy细胞系、对照组脑组织三者中的表达情况。MTT法检测miR383转染细胞与对照组细胞增殖情况,采用流式细胞仪检测miR383转染细胞与对照组细胞凋亡情况。结果人髓母细胞瘤组织、Daoy细胞系、对照组脑组织三者中PRDX3的灰度值与β内参照计算比值,差异有统计学意义(P <0.05),表示三组细胞中PRDX3的表达水平有差异。人髓母细胞瘤组织中PRDX3的表达水平高于对照组脑组织,差异有统计学意义(P<0.05);Daoy细胞系中PRDX3的表达水平高于对照组脑组织,差异有统计学意义(P <0.05)。miR383转染组在转染48 h及72h后,PRDX3的表达水平低于Control组,差异有统计学意义(P <0.05);miR383转染组的Daoy细胞系增殖能力低于Control组水平,差异有统计学意义(P <0. 05);在第3天时,差异最为显著。miR383转染组细胞凋亡比例高于Control组,差异有统计学意义(P <0.05)。结论 PRDX3在人髓母细胞瘤组织、Daoy细胞系中存在过表达情况,而miR383可能通过下调PRDX3的表达水平来抑制Daoy细胞系的增殖,并促进其凋亡。Objective To investigate the effects of miR383 on PRDX3 expression and how this regulation affects proliferation and apoptosis in human medulloblastoma. Methods Fresh tissue samples were collected from 35 patients with medulloblastoma in a hospital from 2015 to2017 ,and 10 other non- tumor brain tissues from the pathology department of our hospital were used as the control group.The human myeloma cell line Daoy was purchased from the cell resources center of Chinese Academy of Sciences.Western blot was used to detect the expression of PRDX3 in three human bone marrow blastoma tissues,Daoy cell lines and control brain tissues.MTT assay was used to detect the proliferation of miR383 transfected cells and control cells.Flowcytometry was used to detect the apoptosis of miR383 transfected cells and control groups. Results The gray values of PRDX3 among human marrow blastoma tissue,Daoy cell line and control group were compared by dividing the beta internal reference,and the difference was significantly different ( P < 0.05),indicating that the expression levels of PRDX3 in the three groups were different.The expression of PRDX3 in the tissue of human marrow blastoma was higher than that of the control group ( P <0.05),and the expression of PRDX3 in the Daoy cell line was higher than that of the control group ( P <0.05).The expression level of PRDX3 in the transfected group was lower than that of control group after 48 hours and 72 hours of transfection ( P <0.05).The proliferation ability of Daoy cell lines in miR383 transfected group was lower than that of the control group,( P <0.05).The difference was the most significant at the day 3 .The percentage of apoptotic cells in miR383 transfection group was higher than that in the control group ( P <0.05). Conclusion PRDX3 is overexpressed in human marrow blastoma tissue and Daoy cell line,and miR383 may inhibit the proliferation of Daoy cell lines and promote the apoptosis by down- regulating the expression level of PRDX3.

关 键 词：miR383 PRDX3 人髓母细胞瘤 细胞凋亡 

分 类 号：R739.4[医药卫生—肿瘤]