ESR技术应用于逆胶束酶体系性能的研究  

Study of enzyme-reverse micelle systembyelectron spin resonance

作　　者：彭馨 徐鹏飞 汤宇 杜浩 袁露 孟勇 PENG Xin;XU Peng-fei;DU Hao;TANG Yu;YUAN Lu;MENG Yong(College of Chemistry and Chemical Engineering,Hunan Normal University,Changsha 410081,China)

机构地区：[1]湖南师范大学化学化工学院石化新材料与资源精细利用国家地方联合工程实验室湖南师范大学化学生物学及中药分析教育部重点实验室有机功能分子组装与应用湖南省重点实验室,湖南长沙410081

出　　处：《中国环境科学》2019年第2期818-822,共5页China Environmental Science

基　　金：国家自然科学基金资助项目(51608194);湖南师范大学化学生物学及中药分析教育部重点实验开放基金资助项目(KLCBTCMR18-12)

摘　　要：采用鼠李糖脂构建逆胶束体系,并利用电子自旋共振(ESR)技术研究了鼠李糖脂逆胶束及鼠李糖脂逆胶束酶体系的性能.采用ESR光谱技术计算得出逆胶束体系中超精细分裂常数变化,研究表明鼠李糖脂在正己烷中的临界胶度为0.07mmol/L.通过分析ESR光谱中旋转相关时间的变化,探究电子自旋探针在逆胶束中运动受阻和翻转所需时间的情况同时,ESR光谱的峰值体现了样品中自由基含量的多少.通过对比逆胶束体系、逆胶束酶体系以及逆胶束酶-苯酚体系的ESR光谱,结果表明鼠李糖脂逆胶束-苯酚体系的自由基最多.通过研究对16-氮氧自由基硬脂酸对两个体系作用,推测出电子自旋探针自由基团定位于逆胶束水核中,并且推断电子自旋探针位于水核的边缘区域,即结合水水层.该研究为逆胶束酶体系的应用提供了坚实的理论基础.In this study,a reverse micelle system was constructed using rhamnolipid,and the properties of rhamnolipid reverse micelles and rhamnolipid reverse micelle enzyme system were studied by electron spin resonance(ESR)technique.ESR spectroscopy was used to calculate the performance.The ultrafine splitting constant in the reverse micelle system was obtained.The study showed that the criticality of rhamnolipid in n-hexane was 0.07mmol/L.By analyzing the change of rotation correlation time in ESR spectrum,the electron spin probe was explored.At the same time,the peak of the ESR spectrum reflects the amount of free radicals in the sample.By comparing the reverse micelle system,the reverse micelle enzyme system and the reverse micelle enzyme-phenol system ESR spectra showed that the rhamnolipid reversed micelle-phenol system had the most free radicals.By studying the effect of 16-nitroxyl radical stearic acid on two systems,it was speculated that the electron spin probe free radicals were localized.In the reverse micelle water core,and inferred that the electron spin probe was located in the edge region of the water core,that was,the water-water layer was combined.This study provided a solid theoretical basis for the application of the reverse micelle enzyme system.

关 键 词：鼠李糖脂 逆胶束 电子自旋共振 探针 16-氮氧自由基硬脂酸 

分 类 号：X132[环境科学与工程—环境科学]