伏立诺他协同PI3K抑制剂NVP-BEZ235诱导Jurkat细胞凋亡  

Induction of apoptosis in Jurkat cells by vorinostat combination with PI3K inhibitor NVP-BEZ235

作　　者：陈明 李君君[1] 肖红[2] 黄力君[2] CHEN Ming;LI Junjun;XIAO Hong;HUANG Lijun(Department of Hematology,the First Affiliated Hospital of Nanhua University,Hengyang 421001,China;Department of Hematology,Xiangtan First People's Hospital in Hu'nan Province,Xiangtan 411101,China)

机构地区：[1]南华大学附属第一医院血液科,湖南衡阳421001 [2]湖南省湘潭市第一人民医院血液科,湖南湘潭411101

出　　处：《中国现代医生》2019年第2期29-31,共3页China Modern Doctor

基　　金：湖南省自然科学基金(2018JJ3433)

摘　　要：目的探讨伏立诺他联合PI3K抑制剂NVP-BEZ235对Jurkat细胞凋亡的影响,并探讨其作用机制。方法体外培养Jurkat细胞,用不同浓度伏立诺他或(和)NVP-BEZ235孵育后,采用MTS观察两者单独用药或联合用药对细胞的增殖的影响,流式细胞术检测细胞凋亡;Western blot检测PI3K/Akt磷酸化及Bim1表达。结果0.12、0.24、0.36、0.48和0.60μmol/L伏立诺他和6、12、18、24、30 nmol/L NVP-BEZ235对Jurkat细胞的生长增殖具有协同作用。伏立诺他或(和)NVP-BEZ235也可促进Jurkat细胞凋亡,并抑制PI3K/Akt磷酸化,上调Bim1的表达。结论伏立诺他对PI3K抑制剂NVP-BEZ235抑制Jurkat细胞生长具有协同作用,并能诱导Jurkat细胞凋亡,其机制与影响Bim1有关。Objective To investigate the effect of vorinostat combined with PI3K inhibitor NVP-BEZ235 on apoptosis of Jurkat cells and to explore its mechanism.Methods Jurkat cells were cultured in vitro,and after incubation with different concentrations of vorinostat or(and)NVP-BEZ235,MTS was used to observe the effect of the two drugs alone or in combination on cell proliferation.And apoptosis was detected by flow cytometry.The PI3K/Akt phosphorylation and Bim1 expression were detected by Western blot.Results The 0.12,0.24,0.36,0.48 and 0.60μmol/L vorinostat and 6,12,18,24,30 nmol/L NVP-BEZ235 had synergistic effects on the growth and proliferation of Jurkat cells.Vorinostat or(and)NVP-BEZ235 also promoted Jurkat cell apoptosis and inhibited PI3K/Akt phosphorylation,up-regulated Bim1 expression.Conclusion Vorinostat has a synergistic effect on the inhibition of Jurkat cell growth by PI3K inhibitor NVP-BEZ235,and can induce apoptosis of Jurkat cells.The mechanism is related to the influence of Bim1.

关 键 词：伏立诺他 急性T淋巴细胞白血病 PI3K抑制剂 NVP-BEZ235 JURKAT细胞凋亡 

分 类 号：R737.9[医药卫生—肿瘤]