经眶下神经注射ET-1复制三叉神经痛大鼠模型  

Study of trigeminal neuralgia in an animal model produced by administration of endothelin-1 to the infraorbital nerve

作　　者：曹启旺[1] 徐慧巧 Qi-wang Cao;Hui-qiao Xu(Department of Pain Medicine,Hunan Provincial People’s Hospital,Changsha,Hunan 410005,China)

机构地区：[1]湖南省人民医院疼痛科,湖南长沙410005

出　　处：《中国现代医学杂志》2019年第4期14-17,共4页China Journal of Modern Medicine

基　　金：湖南省卫生计生委项目(No:C2016014)

摘　　要：目的经眶下神经注射内皮素-1(ET-1)复制三叉神经痛大鼠模型。方法实验1,将24只SD大鼠随机分为A、B、C 3组,A、B组分别经眶下神经注射不同剂量的ET-1溶液,C组注射10μl生理盐水,观察各组大鼠给药后60 min眶下神经支配区域机械痛阈值和抓脸行为的变化。实验2,将16只SD大鼠随机分为两组,分别连续喂给卡马西平(D组)、生理盐水(E组)3 d,然后按实验1的方法复制三叉神经痛模型,观察注射ET-1溶液后60 min大鼠眶下神经支配区域机械痛阈值的变化。结果实验1中与C组比较,A、B组术后60 min大鼠眶下神经支配区域机械痛阈值降低,抓脸行为次数增多,且B组变化更显著(P <0.05)。实验2中D组大鼠术后对机械刺激的痛敏反应敏感性较E组降低(P<0.05)。结论经眶下神经注射ET-1能复制理想的三叉神经痛大鼠模型。Objective To establish a rat model of trigeminal neuralgia produced by administration of endothelin-1(ET-1)to the infraorbital nerve.Methods Experiment 1:Twenty-four healthy Sprague-Dawley rats randomly divided into 3 groups:ET-1 8 nmol injection group(group A,n=8),ET-1 16 nmol injection group(group B,n=8),and saline 10μl injection group(group C,n=8).The changes of mechanical pain threshold and the facial scratching behavior in infraorbital nerve innervation area had been observed during 60 minutes after surgery.Experiment 2:Sixteen healthy Sprague-Dawley rats randomly divided into carbamazepine fed group(group D,n=8)and saline fed group(group E,n=8).To establish model of trigeminal neuralgia according to the method of experiment 1 after continuous feeding for 3 days.The changes of mechanical pain threshold and the facial scratching behavior in infraorbital nerve innervation area had been observed during 60 minutes after surgery.Results Experiment 1:Compared with group C,the mechanical pain threshold of infraorbital nerve innervation area decreased and the number of facial scratching behavior increased in group A and B(P<0.05),and the changes in group B were more significant(P<0.05).Experiment 2:Compared with group E,the mechanical pain threshold of infraorbital nerve innervation area increased and the number of facial scratching behavior decreased in group D(P<0.05).Conclusions Rat model of trigeminal neuralgia can be successfully established by injecting ET-1 into infraorbital nerve.

关 键 词：三叉神经痛 内皮素-1/受体 内皮素A 大鼠 模型 动物 

分 类 号：R745.11[医药卫生—神经病学与精神病学]