沙棘干乳剂对小鼠食管上皮内瘤变的阻断作用初探  

Blocking effect of seabuckthorn emulsion on esophageal intraepithelial neoplasia in mice

作　　者：陈星宇 孔令斌[2] 张帆[2] 方萧 张永超 杨景玉[3] Chen Xingyu;Kong Lingbin;Zhang Fan;Fang Xiao;Zhang Yongchao;Yang Jingyu(Graduate School,Jining 272067;Clinical College of Jining Medical University;Shandong Digestive Disease Prevention Center of Shandong Academy of Medical Sciences,Jining 272033,China)

机构地区：[1]济宁医学院,在读研究生272067 [2]济宁医学院临床学院,272067 [3]山东省医学科学院山东省消化病防治中心,济宁272033

出　　处：《中华诊断学电子杂志》2019年第1期54-58,共5页Chinese Journal of Diagnostics(Electronic Edition)

基　　金：济宁市科技局资助项目(2015130)

摘　　要：目的建立化学致癌剂4-硝基喹啉-1-氧化物(4NQO)诱导的小鼠食管癌前病变模型,探讨沙棘干乳剂对食管上皮内瘤变的阻断作用。方法选取145只健康昆明雄性小鼠,按照随机数字表法分为A、B、C、D 4组,其中A组(空白对照组,n=10,正常饮食、饮蒸馏水,不施加干预)、B组(4NQO诱癌组,n=45)、C组(沙棘干乳剂治疗组,n=45)和D组(全反式维甲酸组,n=45)。B、C、D 3组均于实验开始第1天至第14周末,自由饮用浓度0. 1 g/ml的4NQO水溶液,直至第14周末时小鼠食管癌前病变模型建立成功,B、C和D组分别给予蒸馏水、沙棘干乳剂和全反式维甲酸灌胃,分别于第19周末处死解剖小鼠A组2只,B组、C组和D组小鼠各20只,第24周末处死解剖小鼠A组2只、B组16只、C组25只和D组25只,通过苏木精-伊红(HE)染色和病理组织学检测方法观察并比较各组小鼠食管上皮黏膜组织变化情况,分析沙棘干乳剂对小鼠食管上皮内瘤变的作用。结果小鼠喂养19周前B组、C组和D各组小鼠食管上皮黏膜组织增生变化不明显。24周后C组和D组小鼠食管高级别上皮内瘤变率分别为60. 00%(15/25)和52. 00%(13/25),两组比较差异无统计学意义(χ~2=0. 85,P>0. 05),但均明显低于B组93. 75%(15/16)(χ~2=3. 97,4. 22;均P <0. 05); C组和D组小鼠食管低级别上皮内瘤变率分别为40. 00%(10/25)和48. 00%(12/25),两组比较差异无统计学意义(χ~2=2. 68,P>0. 05),均明显高于B组6. 25%(1/16)(χ~2=5. 66,7. 86;均P <0. 05)。结论沙棘干乳剂对食管上皮内瘤变有一定的阻断作用,可阻断其向高级别瘤变发展,但还不能明确沙棘干乳剂对上皮内瘤变有治疗或逆转作用。Objective To establish a mouse model of esophageal precancerous lesion induced by chemical carcinogen 4-nitroquinoline-1-oxide(4NQO),and to investigate the blocking effect of seabuckthorn emulsion on esophageal intraepithelial neoplasia.Methods One hundred and forty-five healthy Kunming male mice were randomly divided into four groups:group A(blank control group,n=10,normal diet,drinking distilled water without intervention),group B(4NQO induced cancer group,n=45),group C(seabuckthorn emulsion treatment group,n=45)and group D(all-trans retinoic acid treatment group,n=45).Group B,C and D all drank the 4NQO solution of 0.1 g/ml freely from the first day to the 14th weekend,until the mouse esophageal precancerous lesion model was established successfully.Group B,C and D were given distilled water,seabuckthorn emulsion and all-trans retinoic acid respectively by intragastric administration.At the 19th weekend,2 mice in group A,20 mice in group B,20 mice in group C and 20 mice in group D were executed.At the 24th weekend,2 mice in group A,16 mice in group B,25 mice in group C and 25 mice in group D were executed.Hematoxylin-eosin(HE)staining and histopathological examination were used to observe and compare the changes of esophageal epithelial mucosa in each group of mice,and the effect of seabuckthorn emulsion on esophageal intraepithelial neoplasia in mice was analyzed.Results There were no significant changes in the proliferation of esophageal epithelial tissues among the B,C and D groups before 19 w feeding.After 24 weeks,the esophageal high-grade intraepithelial neoplasia rates in C and D groups were 60.00%(15/25)and 52.00%(13/25),respectively,with no significant difference(χ^2=0.85,P>0.05),which were significantly lower than those in group B[93.75%(15/16)(χ^2=3.97,4.22,all P<0.05)].The esophageal low grade intraepithelial neoplasia rates were 40.00%(10/25)and 48.00%(12/25)in group C and D respectively,with no significant difference between the two groups(χ^2=2.68,P>0.05),which were significantly higher than t

关 键 词：沙棘干乳剂 食管肿瘤 上皮内瘤样病变 阻断 小鼠 

分 类 号：R735.1[医药卫生—肿瘤]