Does wetland watershed land use influence amphibian larval development? A relevant effect of agriculture on biota  

作　　者：GARCíA-MU?OZ Enrique GUERRERO Francisco ARECHAGA Garbi?e PARRA Gema 

机构地区：[1]Departamento de Biología Animal, Biología Vegetal y Ecología, Campus de las Lagunillas [2]Centro de Estudios Avanzados en Ciencias de la Tierra, Campus de las Lagunillas [3]Departamento de Ciencias de la Salud, Campus de las Lagunillas

出　　处：《Journal of Oceanology and Limnology》2019年第1期160-168,共9页海洋湖沼学报（英文）

基　　金：Supported by the Ministerio de Educación y Ciencia(Spain)Project(No.CGL2007-61482;co-funded by the European Regional Development Fund)

摘　　要：Agriculture practices have been widely proposed as the major cause of amphibian population decline. However, the majority of these results have been based on laboratory experiments. The present study was conducted to test the repercussion of wetland sediment provenance on amphibian larval development.Bufo bufo larvae were used in two dif ferent treatments in an outdoor experiment, the ?rst one using sediment from one wetland surrounded by intensive agricultural practices, and the second using sediment with no record of agrochemical uses. A negative ef fect was observed in the agricultural treatment, from subcellular to individual level. The results showed that the sediment from agricultural practices watershed generates a reduction in survival. Furthermore, individuals that developed under this treatment showed a lower total length and a delay in the time necessary to complete metamorphosis; these features are connected with recruitment success. In the same way, biochemical analysis showed high values of lipid peroxidation in metamorphs developing in sediments from an agricultural area. Finally, the results obtained highlight the importance of considering the sediments, not only the water, as origin treatment, allowing us to understand the consequences on amphibian populations that inhabit areas af fected by intensive agriculture.Agriculture practices have been widely proposed as the major cause of amphibian population decline. However, the majority of these results have been based on laboratory experiments. The present study was conducted to test the repercussion of wetland sediment provenance on amphibian larval development.Bufo bufo larvae were used in two dif ferent treatments in an outdoor experiment, the ?rst one using sediment from one wetland surrounded by intensive agricultural practices, and the second using sediment with no record of agrochemical uses. A negative ef fect was observed in the agricultural treatment, from subcellular to individual level. The results showed that the sediment from agricultural practices watershed generates a reduction in survival. Furthermore, individuals that developed under this treatment showed a lower total length and a delay in the time necessary to complete metamorphosis; these features are connected with recruitment success. In the same way, biochemical analysis showed high values of lipid peroxidation in metamorphs developing in sediments from an agricultural area. Finally, the results obtained highlight the importance of considering the sediments, not only the water, as origin treatment, allowing us to understand the consequences on amphibian populations that inhabit areas af fected by intensive agriculture.

关 键 词：AMPHIBIAN DECLINE BUFO BUFO survival ECOTOXICOLOGY agricultural practices 

分 类 号：P[天文地球]