Prefoldin 1对乳腺癌BT474细胞上皮-间充质转化的影响  

Effect of Prefoldin 1 on epithelial mesenchymal transition in breast cancer

作　　者：黄超有 李玺[2] 韩铮 朱珊珊 HUANG Chaoyou;LI Xi;HAN Zheng;ZHU Shanshan(Department of Breast and Thyroid Surgery,Hexian Memorial Hospital of Panyu District,Guangzhou 511400,China)

机构地区：[1]广州市番禺区何贤纪念医院乳腺甲状腺外科,广州511400 [2]中山大学附属第三医院乳腺甲状腺外科,广州510630

出　　处：《实用医学杂志》2019年第1期47-50,共4页The Journal of Practical Medicine

基　　金：2017年度广东省自筹经费类科技计划项目(编号:2017ZC0433)

摘　　要：目的探讨Prefoldin 1(PFDN1)对乳腺癌BT474细胞上皮-间充质转化(EMT)的影响。方法将PFND1基因RNA干扰真核表达载体和空白对照基因表达载体导入BT474细胞,构建转染组及空白转染组,另设未转染组。通过Transwell侵袭实验和迁移实验检测细胞侵袭和迁移能力,采用real-timePCR和western blot检测EMT标志分子E-cadherin、N-cadherin、Vimentin表达水平。结果转染组PFND1mRNA和蛋白表达显著低于其他两组(P <0.05)。转染组细胞侵袭和迁移能力低于其他两组(P <0.05)。转染组E-cadherin表达明显强于其他两组(P <0.05),N-cadherin、Vimentin表达明显低于其他两组(P <0.05)。结论 PFDN1能够促进乳腺癌BT474细胞EMT转化。Objective To observe the effect of Prefoldin 1(PFDN1)on the process of epithelial-mesenchymal transition(EMT)in BT474 cells.Methods RNA interference vector of PFND1 gene and blank vector were transfected into BT474 cells to construct the transfection group and the blank transfection group and the un-transfected group was set up.The cell invasion and migration ability of each group was detected by Transwell invasion experiment and migration experiment.Real-time PCR and western blot were used to detect the expression of EMT markers of E-cadherin,N-cadherin and Vimentin.Results The expression of PFND1 mRNA and protein in the transfection group was significantly lower than that in the other 2 groups(P﹤0.05).The invasion and migration ability in the transfection group were significantly poorer than those in the other 2 groups(P﹤0.05).The expression of E-cadherin in transfection group was significantly stronger than that in the other 2 groups(P﹤0.05),but the expressions of N-cadherin and Vimentin were significantly weaker than those of the other 2 groups(P﹤0.05).Conclusion PFDN1 can promote EMT transformation in BT474 cells.

关 键 词：PREFOLDIN 1 乳腺癌BT474细胞系 上皮-间充质转化 肿瘤侵袭 肿瘤转移 

分 类 号：R737.9[医药卫生—肿瘤]