单纯疱疹病毒1型US11蛋白结合DNA甲基转移酶的作用研究3A  

Study on US11 protein binding DNA methyltransferase 3A of herpes simplex virus type 1

作　　者：钟霞 潘建华[1] 童坚[2] ZHONG Xia;PAN Jianhua;TONG Jian(Haikou People′s Hospital,Department of Clinical Laboratory,Haikou 570208,China)

机构地区：[1]海口市人民医院检验科,海口570208 [2]海南医学院附属医院外科,海口570102

出　　处：《实用医学杂志》2019年第1期56-60,共5页The Journal of Practical Medicine

摘　　要：目的通过免疫共沉淀联合质谱分析筛查与单纯疱疹病毒1型(HSV-1)中US11蛋白存在潜在相互作用的宿主蛋白。方法使用荧光标记的HSV-1病毒感染人成纤维细胞(HFF),通过免疫共沉淀(IP)方法对US11及其相互作用蛋白进行提取和纯化,并与质谱(MS)联用来鉴定相关蛋白。结果利用IP-MS分析技术成功筛选出7个与US11存在潜在相互作用宿主蛋白,其中DNA甲基转移酶3A(DNMT3A)被定性为一种新的US11结合蛋白。结论 HSV-1病毒US11蛋白与哺乳动物DNA甲基转移酶机制的功能关联,DNMT3A是HSV-1感染所需的有效宿主因子。Objective To screen host proteins potentially interacting with US11 protein in herpes simplex virus type 1(HSV-1)by immunoprecipitation combined with mass spectrometry.Methods The HFF was infected with the fluorescently labeled HSV-1 virus,and US11 and its interacting protein was extracted and purified by co-immunoprecipitation,and mass spectrometry was performed to identify the related protein.Result IP-MS analysis technology was used to successfully screen 7 host protein with potential interaction with US11,DNA methyltransferases 3A was identified as a new US11 binding protein.Conclusion Our results highlight a functional association of virus US11 protein with the mammalian DNA methyltransferase machinery,indicating DNMT3A as a host factor required for effective HSV-1 infection.

关 键 词：免疫共沉淀 质谱 单纯疱疹病毒1型 US11蛋白 DNA甲基转移酶3A 

分 类 号：R446.6[医药卫生—诊断学] R752.11[医药卫生—临床医学]