枯草芽孢杆菌8-32产抗真菌物质发酵条件优化  

Optimization of fermentation conditions for antifungal substances produced by Bacillus subtilis 8-32

作　　者：贾田惠 杨可欣 时向哲 王欢 刘雪娇[1] 高同国[1] 朱宝成[1] JIA Tianhui;YANG Kexin;SHI Xiangzhe;WANG Huan;LIU Xuejiao;GAO Tongguo;ZHU Baocheng(College of Life Sciences,Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北农业大学生命科学学院,河北保定071000

出　　处：《河北农业大学学报》2019年第1期77-82,89,共7页Journal of Hebei Agricultural University

基　　金：河北省教育厅拔尖人才项目(BJ2016029);河北自然科学基金青年项目(C2015204031);河北农业大学大学生创新创业训练计划资助项目(2018156)

摘　　要：为提高枯草芽孢杆菌菌株8-32抗真菌物质的产量,在单因素试验基础上,采用Plackett-Burman设计法找出影响菌株Bacillus subtilis 8-32产抗真菌物质的主要因素为培养时间、装液量和可溶性淀粉含量。采用最陡爬坡试验逼近最大响应区域,运用Box-Behnken试验设计及响应面分析法对3个显著影响因子的最佳配比进行优化,得到最佳发酵工艺参数为培养时间72.28h,装液量59.80mL/250mL三角瓶,可溶性淀粉含量1.51%。优化后抑菌圈直径从试验设计初期的14.21mm提高至24.51mm。In order to increase the yield of antifungal substances of Bacillus subtilis 8-32,Plackett-Burman method was used on the basis of single factor experiment,and the result showed that the main factors influencing the produce of antifungal substances were culture time,amount of liquid and soluble starch content.The steepest ascent experiment was used to approach the maximum response value,and the optimum ratio of 3 significant factors was optimized by Box-Behnken test design and response surface analysis.The optimum fermentation process parameters were 72.28 h culture time,59.80 mL/250 mL triangle bottle and 1.51%soluble starch.After optimization,the diameter of the inhibition zone increased from 14.21 mm at the beginning of the trial design to 24.51 mm.

关 键 词：大豆根腐病 尖孢镰刀菌 枯草芽孢杆菌8-32 发酵条件 

分 类 号：S565.1[农业科学—作物学]