不同物理诱变方式对休哈塔假丝酵母产乙醇的影响  

Effects of different physical mutagenesis methods on ethanol production by Candida albicans

作　　者：张云野 王轶男 凌宏志[1,2] 宋刚 平文祥[1,2] 葛菁萍 ZHANG Yunye;WANG Yinan;LING Hongzhi;SONG Gang;PING Wenxiang;GE Jingping(Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150080, China;Heilongjiang University Key Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, China)

机构地区：[1]黑龙江大学农业微生物技术教育部工程研究中心,黑龙江哈尔滨150080 [2]黑龙江大学生命科学学院黑龙江省普通高校微生物重点实验室,黑龙江哈尔滨150080

出　　处：《中国酿造》2019年第2期86-92,共7页China Brewing

基　　金：国家自然科学基金项目(31570492)

摘　　要：采用不同物理方式(紫外线、He-Ne激光和微波)对休哈塔假丝酵母(Candida albicans)进行诱变,结合致死率、正突变率、乙醇产量等试验,分析不同诱变因子对该菌株发酵木糖的影响。结果表明,三种物理诱变对菌株诱变能力有差别,具有代表性的紫外线突变株(ZW-6)、He-Ne激光突变株(HN-3)及微波突变株(WB-3)的乙醇产量分别为17.44 g/L、18.49 g/L和18.11 g/L,较原始菌株分别提高13.91%、20.77%和18.29%;乙醇得率分别为0.35 g/g、0.38 g/g和0.37 g/g,较原始菌株分别提高13.80%、23.17%和19.50%。与紫外线及微波各诱变菌株相比,He-Ne激光诱变菌株的正突变率高,激光照射10 min时,正突变率高达48.62%;He-Ne激光诱变菌株的木糖利用率、乙醇产量、乙醇得率均提高最多,提高的范围最大,分别较原始菌株提高1.69%～44.77%、13.12%～40.59%、29.73%～47.75%。因此,确定He-Ne激光为最佳物理诱变因子。Candida albicans was mutated by different physical methods (ultraviolet light, He-Ne laser and microwave), and combined with lethality, positive mutation rate, ethanol yield and other test results, the effects of different mutagenic factors on xylose fermentation were analyzed. The results showed that the three kinds of physical mutagenesis had different mutagenic ability, and the ethanol yields of representative UV mutants (ZW-6), He-Ne laser mutants (HN-3) and microwave mutants (WB-3) were 17.44 g/L, 18.49 g/L, and 18.11 g/L, respectively, which were 13.91%, 20.77%, and 18.29% higher than the original strain. The ethanol yields of the three strains were 0.35 g/g, 0.38 g/g, and 0.37 g/g, respectively, which were 13.80%, 23.17%, and 19.50% higher than the original strain, respectively. Compared with the mutagenized strains of ultraviolet and microwave, the positive mutation rate of He-Ne laser mutagenized strain was high, and the positive mutation rate was as high as 48.62% after laser irradiation for 10 min;the xylose utilization rate, ethanol production and yield of He-Ne laser mutagenized strain increased most, which was 1.69%-44.77%, 13.12%-40.59%, 29.73%-47.75% higher than the original strain, respectively. Therefore, it was determined that the He-Ne laser was the optimal physical mutagenesis factor.

关 键 词：休哈塔假丝酵母 物理诱变 乙醇产量 

分 类 号：TQ223.12[化学工程—有机化工]