黄连素对溃疡性结肠炎大鼠的治疗作用及其机制  

Therapeutical effect of berberine on ulcerative colitis rats and its mechanism

作　　者：张君红[1] 李洪梅 黄雪[1] 井洁[1] 张湘莲[1] 余明华 石榴 ZHANG Junhong;LI Hongmei;HUANG Xue;JING Jie;ZHANG Xianglian;YU Minghua;SHI Liu(The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学第一附属医院,南宁530021

出　　处：《山东医药》2019年第5期40-43,共4页Shandong Medical Journal

基　　金：广西中医药民族医药自筹经费科研课题(GZZC15-29)

摘　　要：目的观察黄连素对溃疡性结肠炎(UC)大鼠的治疗作用,并分析其作用机制。方法将24只SD大鼠随机分成正常组、模型组、黄连素组各8只。模型组和黄连素组给予5. 5%葡聚糖硫酸钠(DSS)溶液自由饮用制作UC模型,正常组给予蒸馏水自由饮用,连续7 d。造模成功后,正常组和模型组给予蒸馏水灌胃,黄连素组给予黄连素100 mg/kg灌胃,连续7 d。观察各组疾病活动度(DAI评分)和结肠组织病理学(病理学评分)变化,ELISA法检测血清IL-9水平,RT-PCR法检测结肠组织中Toll样受体2(TLR2) mRNA表达,SP免疫组化法检测结肠组织中TLR2蛋白表达。结果与正常组比较,模型组、黄连素组DAI评分、结肠组织病理学评分、血清IL-9水平、结肠组织中TLR2 mRNA及TLR2蛋白表达均升高(P均<0. 01)。与模型组比较,黄连素组第14天DAI评分、结肠组织病理学评分下降,血清IL-9水平下降,结肠组织中TLR2 mRNA及TLR2蛋白表达下降(P均<0. 05)。结论黄连素能够改善UC大鼠消化道症状,并促进肠道黏膜愈合,对UC大鼠具有治疗作用;其机制可能是通过降低结肠组织中TLR2 mRNA、TLR2蛋白的表达,从而降低炎症因子水平发挥治疗作用。Objective To investigate the effect and mechanism of berberine in the treatment of ulcerative colitis(UC)in rats.Methods Twenty-four SD rats were divided into three groups(n=8):the control group,model group,and berberine group.The rats in the model group and berberine group were given 5.5%dextran sulfate sodium(DSS)solution to drink freely,forming the UC rat models.The rats in the control group were given distilled water to drink freely for seven days.In the next seven days,the control group and the model group were given distilled water by gavage,and the berberine group was given berberine(100 mg/kg).The degree of disease activity(DAI score)and the pathological changes of colon tissues in each group were observed.The serum IL-9 level was measured by ELISA,and the reverse transcription polymerase chain reaction(RT-PCR)and immunohistochemistry were used to detect the expression levels of TLR2 mRNA and TLR2 protein in rat colon tissues.Results Compared with the control group,the DAI score,the colon histopathology score,the serum IL-9 level,TLR2 mRNA and TLR2 protein expression increased in the model group and berberine group(all P<0.01).Compared with the model group,the DAI score on day 14 and the colon histopathology score decreased,and the serum IL-9 level,mRNA and protein expression of TLR2 decreased in the berberine group(all P<0.05).Conclusions Berberine improves gastrointestinal symptoms and promotes intestinal mucosal healing in rat with UC.Berberine may play an important role in the treatment of UC by reducing the level of IL-9 and the mRNA and protein expression of TLR2.

关 键 词：溃疡性结肠炎 黄连素 白细胞介素9 TOLL样受体2 大鼠 

分 类 号：R574.62[医药卫生—消化系统]