丹参酮ⅡA通过抑制STAT3活化调控胃癌细胞增殖和凋亡的研究  

Study on tanshinoneⅡA regulates proliferation and apoptosis of gastric cancer cells via inhibition of STAT3 activation

作　　者：张拥军[1] 彭伯坚[1] 曹天生[2] 卢秀珊 郭曙光[3] ZHANG Yongjun;PENG Bojian;CAO Tiansheng;LU Xiushan;GUO Shuguang(Department of Gastroenterology;Department of General Surgery,Huadu District People′sHospital,Southern Medical University,Guangzhou,Guangdong 510800,China;Physical ExaminationCenter,The Third Affiliated Hospital,Sun Yat-sen University,Guangzhou,Guangdong 510006,China)

机构地区：[1]广东省广州市花都区人民医院消化内科,510800 [2]广东省广州市花都区人民医院普外科,510800 [3]中山大学附属第三医院体检科,广州510630

出　　处：《重庆医学》2019年第4期559-563,共5页Chongqing medicine

基　　金：广州市花都区科技工业和信息化局--医疗卫生一般科研专项项目(17-HDWS-021)

摘　　要：目的研究丹参酮ⅡA (TanⅡA)对胃癌细胞增殖的抑制作用,并探讨其分子机制。方法应用四甲基偶氮唑蓝(MTT)法检测不同浓度的TanⅡA(2.5、5、10μg/mL)对3种胃癌细胞株(SNU-638、MKN1和AGS)增殖的影响;流式细胞仪检测细胞凋亡情况;Western blot检测凋亡相关蛋白(Bax、cleaved caspase-3、Bcl)以及信号转导和转录激活因子3(STAT3)、p-STAT3蛋白表达变化;荧光素酶报告基因检测STAT3转录活性变化。结果与对照组比较,TanⅡA能明显抑制胃癌细胞的增殖,促进细胞凋亡,增加凋亡相关蛋白Bax和cleaved caspase-3的表达,降低Bcl的表达;TanⅡA明显抑制STAT3磷酸化,过表达STAT3可下调TanⅡA的促凋亡作用及对STAT3转录活化的抑制作用。结论 TanⅡA可能通过抑制STAT3转录活化调控胃癌细胞的增殖和凋亡。Objective To investigate the effect and molecular mechanism of tanshinone ⅡA (TanⅡA) on proliferation and apoptosis of gastric cancer cells. Methods The MTT assay was performed to determine the effect of TanⅡA (2.5,5,10 μg/mL) on cell proliferation in SNU-638,MKN1 and AGS gastric cancer cells.The flow cytometry was applied to detect the cellular apoptosis;the expression changes of apoptosis related proteins( Bax,cleaved caspase-3,Bcl) and STAT3,p-STAT3 protein were detected by Western blot.The luciferase reporter assay was used to determine the change of STAT3 transcriptional activity. Results Compared with the control group,TanⅡA could significantly inhibit the cell proliferation in gastric cancer cells,promoted the cellular apoptosis,increased apoptosis related protein Bax and cleaved caspase-3 protein expression,and reduced the Bc1 expression;TanⅡA significantly inhibited STAT3 phosphorylation,over expressed STAT3 could down-regulate the TanⅡA apoptosis-promoting effect and inhibited the STAT3 transcription activation. Conclusion TanⅡA regulates the proliferation of gastric cancer cells through inhibiting STAT3 transcription activation.

关 键 词：胃肿瘤 丹参酮ⅡA 信号转导和转录激活子3 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]