通过烟熏和间歇低氧构建大鼠COPD-OSAHS重叠综合征模型  

Establishment of a rat model of COPD-OSAHS overlap syndrome induced by smoking and intermittent hypoxia

作　　者：陈敏[1] 黄照明 何剑 毕虹[1] 王清[1] 何乐伟 杜俊毅[1] 周开华[1] 金志贤[1] CHEN Min;HUANG Zhaoming;HE Jian;BI Hong;WANG Qing;HE Lewei;DU Junyi;ZHOU Kaihua;JIN Zhixian(Department of Pneumology, the First People’s Hospital-Calmette Hospital of Kunming, Kunming 650224, China)

机构地区：[1]昆明市第一人民医院甘美医院呼吸与危重症医学科,昆明650224

出　　处：《中国实验动物学报》2019年第1期59-64,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金资助项目(81560010)~~

摘　　要：目的构建大鼠COPD-OSAHS重叠综合征(OS)模型,为开展OS基础研究提供可行手段及实验平台。方法将14只SPF级雌性6周龄SD大鼠随机分为实验组和对照组,其中实验组(8只)予烟熏和间歇低氧暴露,对照组(6只)予假烟熏和正常氧暴露。8周后计算实验组大鼠的存活率,并对比两组大鼠的一般情况、血气分析、右心室肥厚指数、平均肺泡数、平均肺泡间隔、肺组织病理改变。结果间歇低氧箱氧浓度-时间变化曲线显示氧浓度均周期性降低及恢复正常,成功模拟出间歇低氧气体环境;实验组最终存活6只,存活率为75%,对照组6只大鼠全部存活。与对照组相比,实验组6只存活的SD大鼠一般情况较差,存在酸中毒、低氧血症、二氧化碳潴留、右心室肥厚、平均肺泡截距明显增大、平均肺泡数明显减少(P<0.05),差异有统计学意义;肺组织病理观察显示肺间质炎症浸润、支气管壁淋巴细胞增生、细支气管管壁平滑肌增生、管壁平滑肌部分断裂、杯状细胞增生及肺气肿。结论采用烟熏联合间歇低氧暴露方法成功建立了大鼠OS模型,更加有意义及客观的模型评价指标有待研究发现。Objective To establish a rat model of chronic obstructive lung disease (COPD) and obstructive sleep apnea-hypopnea synclrome(OSAHS) overlap syndrome (OS), which can provide a feasible means and experimental platform for basic research on the overlap syndrome. Methods14 SPF female 6-week-old SD rats were randomly divided into experimental group ( n =8) and control group ( n =6). The rats in experimental group were exposed to smoke and intermittent hypoxia environment, and the rats in control group were given sham smoke and normal oxygen. After 8 weeks, the survival rate of rats in experimental group was calculated, and the general condition, blood gas analysis, right ventricular hypertrophy index, mean alveolar numbers, mean alveolar septum, and pathological changes of lung tissue were compared between the two groups. ResultsThe oxygen concentration-time curve of intermittent hypoxic chamber showed that oxygen concentration decreased periodically and returned to normal, which indicated that the intermittent hypoxic gas environment was successfully simulated. At last, 6 rats in the experimental group survived, with a survival rate of 75%, while all rats in control group survived. Compared with the control group, the 6 surviving SD rats in experimental group were generally in poor condition, with acidosis, hypoxemia and carbon dioxide retention, right ventricular hypertrophy, significantly increased mean alveolar intercept and significantly decreased mean alveolar number ( P < 0.05). The difference was statistically significant. Pathological observation of lung tissue showed that pulmonary interstitial inflammatory infiltration, lymphocyte proliferation in bronchial wall, smooth muscle hyperplasia in bronchial wall, partial rupture of smooth muscle in bronchial wall, goblet cells hyperplasia and emphysema. Conclusions A rat model of OS is successfully established by smoking and intermittent hypoxia exposure. More meaningful and objective evaluation indicators of the OS model are still to be found.

关 键 词：肺气肿 烟熏 间歇低氧 睡眠呼吸暂停 重叠综合征 模型 大鼠 

分 类 号：Q95-33[生物学—动物学]