Wnt7a对肝癌细胞凋亡、迁移及侵袭的影响  

Effects of Wnt7a on hepatocellular carcinoma cell apoptosis,invasion and migration

作　　者：兰莉辉 王巍[2] 邵跃[2] 赵成海[2] 卜献民[1] Lan Lihui;Wang Wei;Shao Yue;Zhao Chenghai;Bu Xianmin(Department of Hepatobiliary and Spleen Surgery,Shengjing Hospital of China Medical University,Liaoning Shenyang 110004,China;Department of Pathophysiology,the College of Basic Medical Science of China Medical University,Liaoning Shenyang 110122,China)

机构地区：[1]中国医科大学附属盛京医院肝胆脾外科,辽宁沈阳110004 [2]中国医科大学基础医学院病理生理学教研室,辽宁沈阳110122

出　　处：《现代肿瘤医学》2019年第5期746-749,共4页Journal of Modern Oncology

基　　金：辽宁省科学技术厅社会攻关及产业化项目(编号:2017225028)

摘　　要：目的:检测Wnt7a在肝癌中的表达,分析Wnt7a对肝癌细胞活性、凋亡、迁移及侵袭的影响,探讨Wnt7a在肝癌中的作用。方法:分别采用免疫组织化学染色和Western blot检测组织和细胞系中Wnt7a的表达情况; Hep3B细胞经人重组Wnt7a蛋白(rW nt7a)处理后,MTT法检测细胞活性改变,流式细胞仪检测细胞凋亡变化,Transwell分析细胞迁移及侵袭能力变化。结果:相对于癌旁组织,肿瘤组织低表达Wnt7a蛋白;经rW nt7a处理后,Hep3B细胞活性降低、细胞凋亡增多且迁移与侵袭能力下降。结论:Wnt7a蛋白能抑制Hep3B细胞生长、迁移与侵袭能力,可能在肝癌中发挥抑癌作用。Objective:To explore the role of Wnt7a in hepatocellular carcinoma by investigating Wnt7a expression in hepatocellular carcinoma and analyzing effects of Wnt7a on Hep3B cell viability,apoptosis,migration and invasion.Methods:Immunohistochemistry and Western blot were applied to detect Wnt7a expression in tissues and cells,respectively.Hep3B cells were treated with recombinant Wnt7a protein(rWnt7a).Cell viability was assayed by MTT.Cell apoptosis was examined by flowcytometry.Cell migration and invasion was analyzed by transwell.Results:Wnt7a was expressed in hepatocellular carcinoma tissues in a lower level compared with cancer adjacent tissues.Treatment of Hep3B cells with rWnt7a reduced cell viability,increased cell apoptosis,and decreased cell migration and invasion.Conclusion:Wnt7a inhibits Hep3B cell growth,migration,and invasion,suggesting Wnt7a may play a tumor suppressing role in hepatocellular carcinoma.

关 键 词：肝癌 Wnt7a 凋亡 迁移 侵袭 

分 类 号：R735.7[医药卫生—肿瘤]