对顺铂耐药胃癌细胞的DNA甲基化表型标志物初筛  

Preliminary screening of cisplatin resistant DNA methylation phenotypic markers in gastric cancer

作　　者：刘洁[1] 彭微[1] 龚宗跃[1] 徐筱红[1] 刘映[1] Liu Jie;Peng Wei;Gong Zongyue;Xu Xiaohong;Liu Ying Hunan(Traditional Chinese Medical College,Hunan Zhuzhou 412000,China)

机构地区：[1]湖南中医药高等专科学校,湖南株洲412000

出　　处：《现代肿瘤医学》2019年第5期754-756,共3页Journal of Modern Oncology

基　　金：湖南省教育厅科技处一般项目(编号:15C1074)

摘　　要：目的:寻找和鉴定对顺铂耐药胃癌细胞的异常甲基化基因。方法:我们拟胃癌细胞和耐药细胞为研究对象,采用DNA甲基化芯片,对比分析两种细胞DNA甲基化表达谱差异,结合甲基化特异性PCR(MSPCR)技术验证获得的异常甲基化基因。结果:利用DNA甲基化谱芯片对胃癌细胞和耐药细胞两个细胞系的基因组DNA甲基化谱进行分析,发现1 095个甲基化差异位点;并筛选出36个高甲基化,14个低甲基化修饰基因。然后通过MS-PCR方法对这50个基因的甲基化修饰在细胞水平上又进行了验证分析,最终筛选出与甲基化谱芯片结果一致的14种基因,包括11种高甲基化和3种低甲基化修饰基因。结论:胃癌癌细胞发生顺铂耐药时,细胞基因组存在广泛的DNA甲基化修饰改变。Objective:To find and identify abnormal methylation genes for cisplatin resistant gastric cancer cells.Methods:Gastric cancer cells and drug-resistant cells as our targets,we used DNA methylation chips to compare and analyze the different expressions of two kinds of DNA methylation cells in order to verify the abnormal methylation genes by means of methylation specific PCR(MS-PCR)technique.Results:The genomic DNA methylation profiles of two cell lines of gastric cancer cells and drug-resistant cells were analyzed by DNA methylation microarray.1 095 methylation loci were identified,and 36 hypermethylation and 14 hypomethylation genes were screened out.Then the methylation of these 50 genes was verified by MS-PCR analysis at the cell level.Finally,14 genes,which were identical to the methylation microarray,were screened out,including 11 hypermethylation and 3 hypomethylation genes.Conclusion:When the cancer cells of gastric cancer occur cisplatin resistance,there is a wide range of DNA methylation modification in the cell genome.

关 键 词：胃癌 DNA甲基化 顺铂 耐药 

分 类 号：R735.2[医药卫生—肿瘤]