异性烯醇化酶和X链锁凋亡抑制蛋白在先天性胆管扩张症发病中的作用  

Heterosexual enolase and X-linked inhibitor of apoptosis protein in the pathogenesis of congenital biliary dilatation

作　　者：吴宙光[1] 王斌[1] 陈子民[1] 冯奇[1] 叶晓烁[1] 王建尧[1] Wu Zhouguang;Wang Bin;Chen Zimin;Feng Qi;Ye Xiaoshuo;Wang Jianyao(Department of General Surgery,Shenzhen Children's Hospital,Guangdong Shenzhen 518026,China)

机构地区：[1]深圳市儿童医院普外科,广东深圳518026

出　　处：《现代肿瘤医学》2019年第5期833-835,共3页Journal of Modern Oncology

基　　金：深圳市卫生计生系统科研项目(编号:201507029)

摘　　要：目的:探索异性烯醇化酶和X链锁凋亡抑制蛋白对先天性胆管扩张症的致病作用,为先天性胆管扩张症发病机制研究提供实验、理论依据。方法:收集先天性胆管扩张症根治术后胆囊标本50例为先天性胆管扩张症组及32例非胆系尸检胆囊标本为对照组,采用酶联免疫吸附测定法(ELISA),对胆囊中异性烯醇化酶和X链锁凋亡抑制蛋白的含量进行测定,应用疼痛分级对表达量与分级标准进行关系分析。结果:对照组胆囊液中异性烯醇化酶和X链锁凋亡抑制蛋白的蛋白表达水平低于先天性胆管扩张症组,差异具有统计学意义(P <0. 05)。随着疼痛分级的上升,异性烯醇化酶和X链锁凋亡抑制蛋白水平表达升高,差异有统计学意义(P <0. 05)。结论:异性烯醇化酶和X链锁凋亡抑制蛋白与先天性胆管扩张症的发病可能有关,并有望成为反应先天性胆管扩张症疼痛严重性的一个生化指标。Objective:To explore the pathogenic role of heterosexual enolase and X-linked inhibitor of apoptosis protein in congenital biliary duct dilatation and to provide experimental and theoretical evidence for the pathogenesis of congenital biliary.Methods:50 cases underwent radical gastrectomy were collected.At the same time,32 cases of control group were collected.Enzyme-linked immunosorbent assay(ELISA)was used to determine the content of heterosexual enolase and X-linked inhibitor of apoptosis protein in the gallbladder.Relationship of the expression levels and grading criteria was analyzed.Results:Compared with congenital biliary duct dilatation group,the protein expression levels of heterosexual enolase and X-linked inhibitor of apoptosis protein in the control group were significantly lower(P<0.05).With the increase of pain grade,the expression of heterosexual enolase and X-linked inhibitor of apoptosis protein increased,and the difference was statistically significant(P<0.05).Conclusion:Heterosexual enolase and X-linked inhibitor of apoptosis protein may be associated with the pathogenesis of congenital protein dilatation and may be biochemical indicators of the severity of pain in congenital biliary dilatation.

关 键 词：异性烯醇化酶 X链锁凋亡抑制蛋白 先天性胆管扩张症 发病机制 

分 类 号：R735.8[医药卫生—肿瘤]