大鼠脊髓挫伤模型对比及行为学评价  

Comparison and behavioral evaluation of rat models of spinal cord contusion

作　　者：唐涛 马小翠 姚冰 陈一 朴浩哲 TANG Tao;MA Xiaocui;YAO Bing;CHEN Yi;PIAO Haozhe(Department of Neurosurgery Cancer Hospital of China Medical University,Liaoning Cancer Hospital & Institute,Shenyang 110042,China;Department of Anesthesiology,Cancer Hospital of China Medical University,Liaoning Cancer Hospital & Institute,Shenyang 110042,China)

机构地区：[1]中国医科大学肿瘤医院辽宁省肿瘤医院神经外科,沈阳110042 [2]中国医科大学肿瘤医院辽宁省肿瘤医院麻醉科,沈阳110042

出　　处：《中国比较医学杂志》2019年第2期84-88,共5页Chinese Journal of Comparative Medicine

摘　　要：目的建立不同程度MASCIS和IH大鼠脊髓损伤模型,研究损伤程度、造模方式与行为、解剖结果之间的关系。方法将80只雌性SD大鼠随机分为4组,每组20只。分别制作NYU 12. 5 mm、NYU 25 mm、IH 150kdyn和IH 200 kdyn模型,对模型制作成功率、BBB评分、Grid Walking评价、热痛以及损伤中心残余组织量进行评价。结果两种造模方法成功率相似;所有动物损伤后0～3 d BBB评分均降至0分左右,随时间增加,BBB评分均逐渐恢复,4～6周达到平台期,NYU 25 mm组评分最低,IH 200组评分居中,IH 150组合NYU 12. 5 mm组评分相似且最高; Grid Walking坠落率随损伤程度增高而增加; Hargreaves结果受运动功能影响较大;运动障碍与损伤中心层面组织残余量成反比。结论 MASCIS和IH模型均能满足脊髓损伤病理生理机制研究要求,但应根据实验目的选取相适应的造模参数。Objective To establish animal models of spinal cord injury of different degrees of severity,namely,MASCIS and IH,and to study the relationship among the degree of damage,modeling mode,behavior,and anatomical results.Methods Eighty female SD rats were randomly and equally divided into four groups:NYU 12.5 mm,NYU25 mm,IH 150 kdyn force,and IH200 kdyn force models.Their rates of successful model establishment,BBB score,grid walking evaluation,Hargreaves test,and total cross-sectional area of the spinal cord at the injury epicenter were evaluated.Results The success rates in the two modeling methods were similar.The BBB scores of all animals were reduced to 0 points at 0 to 3 days after spinal cord injury.As time passed,the BBB scores gradually recovered and reached a plateau at 4 to 6 weeks.The NYU25 mm group scored the lowest,the IH200 group had an intermediate score,and the IH150 and NYU 12.5 mm groups scored similarly and highest.The grid walking score increased with increasing injury severity.The Hargreaves test results were more affected by motor function.Dyskinesia was inversely proportional to the residual amount of tissue at the center of the injury.Conclusions Both MASCIS and IH models are appropriate for research on the pathophysiological mechanisms of spinal cord injury,but appropriate modeling parameters should be selected according to the purpose of the experiment.

关 键 词：脊髓损伤 模型 动物 行为学 

分 类 号：R-33[医药卫生]