转录因子Snail对胃癌MKN-28细胞的增殖、凋亡及侵袭的影响  

Effect of Transcription Factor Snail on Proliferation,Apoptosis and Invasion of Gastric Cancer MKN-28 Cells

作　　者：武平 Wu Ping(Jinci College,Shanxi University of Medical Sciences,Shanxi Taiyuan,030025)

机构地区：[1]山西医科大学晋祠学院,山西太原030025

出　　处：《生物化工》2019年第1期90-93,共4页Biological Chemical Engineering

摘　　要：目的:探讨转录因子Snail对胃癌MKN-28细胞的影响。方法:取胃癌MNK-28细胞株的Snail基因进行沉默研究,以顺铂对MNK-28细胞进行处理,并选用CCK-8法不同刺激因素组细胞的增殖情况进行检测,同时选用透射电镜对各组细胞凋亡情况进行观察,而各组细胞侵袭能力则以Transwell小室进行观察。结果:(1)细胞增殖情况分析,顺铂处理及慢病毒沉默Snail基因均可见细胞增殖抑制现象,而shRNA+Snail+顺铂组细胞增殖抑制率显著高于其他组(p <0.05);(2)细胞凋亡情况,与单纯MKN-28细胞组比较,不同刺激因素组细胞凋亡程度均显著升高(p <0.05);(3)细胞侵袭实验分析,shRNA+Snail+顺铂组细胞迁出的细胞数较其他组明显降低(p <0.05)。结论:转录因子Snail可对胃癌MKN-28细胞的增殖情况产生影响,沉默Snail基因有利于加速细胞凋亡,提高化疗药敏感性,可在临床上推广。Objective:To investigate the effect of transcription factor Snail on gastric cancer MKN-28 cells.Method:The Snail gene of gastric cancer MNK-28 cell line was silenced,MNK-28 cells were treated with cisplatin,and the proliferation of cells with different stimulatory factors was selected by CCK-8 method.The cells of each group were selected by transmission electron microscopy.Apoptosis was observed,and the invasive ability of each group was observed in a Transwell chamber.Result:(1)Analysis of cell proliferation,inhibition of cell proliferation by cisplatin treatment and lentiviral silencing of Snail gene,and inhibition rate of shRNA+Snail+cisplatin group was significantly higher than other groups(p<0.05);(2)Compared with MKN-28 cells alone,the degree of apoptosis was significantly increased in different stimulatory factors(p<0.05).(3)Cell invasion assay,shRNA+Snail+cisplatin cells migrated out.The number of cells was significantly lower than that of the other groups(p<0.05).Conclusion:The transcription factor Snail can affect the proliferation of gastric cancer MKN-28 cells.Silencing the Snail gene is beneficial to accelerate apoptosis and increase the sensitivity of chemotherapeutic drugs,which can be promoted clinically.

关 键 词：转录因子SNAIL 胃癌 MKN-28 细胞 增殖 凋亡 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]