贵州民族药苦皮藤药材质量标准研究  

Quality standards study of Guizhou ethnic medicine Celastrus angulatus Maxim

作　　者：徐艳 成运 何应超 程宽寿 胡奇志 胡成刚 王学军 XU Yan;CHENG Yun;HE Yingchao;CHENG Kuanshou;HU Qizhi;HU Chenggang;WANG Xuejun(Guizhou University of Traditional Chinese Medicine,Guiyang 550002,China;Xi′an Afang Pharmacy Co.Ltd.,Xi′an 710018,China)

机构地区：[1]贵州中医药大学,贵州贵阳550025 [2]西安阿房药业股份有限公司,陕西西安710018

出　　处：《贵州科学》2019年第1期5-9,59,共6页Guizhou Science

基　　金：贵州省食品药品监督管理局项目:南蛇藤根药材质量标准研究;2012年贵州省科技厅中药现代化项目:贵州苗药骚羊古等10种药材质量控制研究;贵州省国内一流建设学科项目(中药学)(GNYL[2017]008号);贵州省中药民族医药科普基地培育(黔科合体K字[2011]4008号)

摘　　要：目的:为贵州民族药材苦皮藤建立一个质量控制方法。方法:采用薄层色谱法对苦皮藤药材中儿茶素进行鉴别;采用高效液相色谱法对药材中儿茶素的含量进行测定,色谱条件:JADE-PAK ODS-AQ色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.5%冰醋酸溶液(7.8∶92.2)为流动相,检测波长280 nm,柱温30℃,流速1.0 mL/min。结果:薄层色谱法鉴别苦皮藤药材的特征斑点清晰,分离度良好;儿茶素在0.12μg～1.56μg范围内线性关系良好,线性回归方程为y=369.51x-1.7732(r=0.9999),精密度及加样回收试验结果符合质量标准研究的要求。结论:该法简便易用,专属性好、结果准确,可用于苦皮藤药材的质量控制。In order to establish a quality control method for Guizhou ethnic medicine Celastrus angulatus Maxim,we adopted thin layer chromatography to identify the catechin in Celastrus angulatus,and high performance liquid chromatography to determine the content of catechin.The HPLC parameters were as follows:JADE-PAK ODS-AQ column(4.6 mm×250 mm,5μm),the mobile phase was acetonitrile-0.5%glacial acetic acid solution(7.8∶92.2),the detection wavelength was 280 nm,the column temperature was 30℃,and the flow rate was 1.0 mL/min.TLC results showed clear characteristic spots and good separation.The linear relationship of catechin whtin the range of 0.12μg to 1.56μg was good,and the linear regression equation was y=369.51x-1.7732(r=0.9999).Precision test and recovery test results met the requirements of quality control standards.This method is simple and easy to operate,has good specificity and accurate results,and can be used for quality control of Celastrus angulatus Maxim.

关 键 词：苦皮藤 质量标准 儿茶素 薄层色谱 高效液相色谱 

分 类 号：R282.71[医药卫生—中药学]