窖泥中高产愈创木酚类功能菌的筛选与鉴定  

Screening and identification of functional boateria with high yield guaiacol in the mud

作　　者：周金虎[1,2,3] 李良 方尚玲 董孝元[4] 张超 陈茂彬 ZHOU Jinhu;LI Liang;FANG Shangling;DONG Xiaoyuan;ZHANG Chao;CHEN Maobin(Key Laboratory of Fermentation Engineering,Ministry of Education,Hubei University of Techonolgy,Wuhan 430068,China;Yellow Crane Tower Wine (Xianning) Co.,Ltd.,Xianning 437000,China;Hubei Collaborative Innovation Center for Industrial Fermentation,Wuhan 430068,China;Hubei Key Laboratory of Industrial Microbiology,Wuhan 430068,China)

机构地区：[1]发酵工程教育部重点实验室(湖北工业大学),湖北武汉430068 [2]工业发酵湖北省协同创新中心,湖北武汉430068 [3]工业微生物湖北省重点实验室,湖北武汉430068 [4]黄鹤楼酒业(咸宁)有限公司,湖北咸宁437000

出　　处：《食品与发酵工业》2019年第3期64-70,共7页Food and Fermentation Industries

基　　金："十三五"国家重点研发计划项目中的重点课题子课题(2016YFD0400500):传统酿造食品风味与品质调控及新型酿造技术创制

摘　　要：试验以黄鹤楼酒浓香型窖泥为材料,采用传统微生物分离方法即稀释涂布平板法对样品进行涂布,然后平板划线纯化、镜检,单菌发酵利用GC-MS筛选出高产愈创木酚类物质的功能菌株,然后利用分子生物学技术对菌种进行鉴定,最后进行菌种的耐受性分析。试验结果为:筛选出2株高产愈创木酚类物质的功能菌B-1、B-3,经鉴定分别为Bacillus amyloliquefaciens、Delftia sp. X-a12,总产量分别为3 632. 33和14 527. 31μg/L。耐受性分析结果为菌株B-1、B-3均耐4%vol酒精度、pH 4. 5、15%糖度。The experiment was carried out using dilution coating method to coat the mud of the Yellow Crane Tower Luzhou-flavor liquor.Plate scribing purification,microscopic examination,and single-bacteria fermentation were conducted,followed by using gas chromatography-mass spectrometer(GC-MS)to screen functional bacteria with high yield of guaiacol.The results of molecular biology techniques showed that two functional strains,B-1 and B-3,with high-yield of guaiacol were Bacillus amyloliquefaciens and Delfitia sp.X-a12,respectively.Their total yields were 3 632.33μg/L and 14 527.31μg/L,respectively.The tolerance analysis results showed that both strains were resistant to 4%vol.alcohol,pH=4.5,and 15%sugar.

关 键 词：戴尔福特菌 解淀粉芽孢杆菌 愈创木酚 菌种鉴定 

分 类 号：TS261.1[轻工技术与工程—发酵工程]