解淀粉芽孢杆菌L-S60与黄瓜互作特性研究  

Characteristics of Interaction between Bacillus amyloliquefaciens L-S60 and Cucumbers

作　　者：张莹[1] 秦宇轩[1] 尚庆茂[2] 张一凡 李昌辉 李平兰[1] ZHANG Ying;QIN Yuxuan;SHANG Qingmao;ZHANG Yifan;LI Changhui;LI Pinglan(College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《农业机械学报》2019年第2期258-265,共8页Transactions of the Chinese Society for Agricultural Machinery

基　　金：国家自然科学基金项目(31671831);公益性行业(农业)科研专项(201303014)

摘　　要：植物根系分泌物作为植物与土壤进行物质交换和信息传递的重要载体物质,对植物-微生物种间互作发挥着重要作用。本文分析了解淀粉芽孢杆菌L-S60与黄瓜的互作过程,研究了黄瓜根系分泌物的主要成分有机酸及氨基酸对菌株的趋化作用及菌株的利用情况,通过计数和荧光定量PCR的方式分析了菌株在黄瓜幼苗的根际定殖情况。结果表明,L-S60能够部分利用黄瓜根系分泌物中主要的有机酸(柠檬酸、草酸、琥珀酸和苹果酸)及氨基酸(谷氨酸、苯丙氨酸、半胱氨酸和色氨酸)成分并且受到这些成分的趋化作用,其中对于苹果酸和谷氨酸的利用情况及受趋化作用最优。从定殖量及相关基因转录水平差异上分析,菌株具有在黄瓜幼苗根系表面定殖的能力,且菌株最大定殖量为1. 02×10~5CFU/g。Plant root exudate plays an important role in the process of material exchange between plant and soil.Moreover,it is a significant factor in‘plant-microorganisms’interactions.The process of interaction between Bacillus amyloliquefaciens L S60 and cucumbers was analyzed.The utilization and chemotaxis of the main components of cucumber root exudate,organic acids and amino acids,by L S60 were studied.After establishing the interaction model between the strain and the cucumber seedlings,the coloning of root surface in hydroponic culture was studied via counting the strain by time.Furthermore,the genes related to root colonization,swarming motility and biofilm formation on translational level were analyzed by fluorescent quantitation PCR.The results showed that L S60 could utilize the main organic acids(citric acid,oxalic acid,succinic acid and malic acid)and amino acids(glutamic acid,phenylalanine,cysteine and tryptophan)from cucumber root exudates and provide well utilization of malic acid and glutamic acid.Also,L S60 showed the chemotaxis toward these components and could be strongly attracted to malic acid and glutamic acid.According to the CFUs,which could colonize on the root surface,and the relative quantitative expression of the related genes on translational level,L S60 showed strong colonization capacities on the cucumber root surface and the most amount of colonization was 1.02×10 5 CFU/g.In conclusion,L S60 showed great potential to become a biological control agent of practical applications in the future.

关 键 词：解淀粉芽孢杆菌 黄瓜 根系分泌物 互作特性 

分 类 号：S482.7[农业科学—农药学]