miR-222及其靶基因BBC3在肝细胞癌组织中的表达及其相关性  

The Expression and Correlation of Mir-222 and Its Target Gene BBC3 in Hepatocellular

作　　者：刘志春[1] 党存曙[1] 刘彬[1] 孙景武[1] 赵梦杰 LIU Zhichun;DANG Cunshu;LIU Bin(Central Hospital of China National Petroleum Corporation,Langfang,065000)

机构地区：[1]河北省廊坊市中国石油天然气集团公司中心医院,065000

出　　处：《实用癌症杂志》2019年第2期200-203,共4页The Practical Journal of Cancer

基　　金：河北省廊坊市科技支撑计划项目(编号:2015013009C)

摘　　要：目的探讨miR-222及其靶基因BBC3在肝癌组织中的表达变化及其相关性。方法选取肝癌和相应癌旁组织各70例,正常肝组织20例,应用RT-PCR方法检测各组miR-222表达水平,应用免疫组织化学技术检测BBC3蛋白表达水平,分析靶基因BBC3表达水平与miR-222的相关性。结果 miR-222 RNA在肝癌患者肝癌组织和癌旁组织中的表达水平明显高于正常对照组,两两比较,差异有统计学意义(P <0. 05);而肝癌组织miR-222 RNA表达水平高于癌旁组织,差异有统计学意义(P <0. 05)。BBC3在肝癌组织、癌旁组织及正常肝组织中的阳性表达率分别为35. 71%、52. 86%、80. 00%,3组之间BBC3表达均有显著差异(P <0. 05)。miR-222与BBC3蛋白表达水平在肝癌组织中呈显著负相关(γ=-0. 497),癌旁组无统计学相关关系。结论 miR-222在肝癌组织中表达明显上调,BBC3在肝癌组织中的表达下调,两者之间为负调控作用,但在癌旁组织中未见明显调控作用,其生物学意义有待进一步研究。Objective To investigate the expression and correlation between miR-222 and its target gene BBC3 in hepatocellular carcinoma.Methods Seventy cases of liver cancer and corresponding adjacent tissues were selected,and 20 cases of normal liver tissues were selected.The expression of miR-222 was detected by RT-PCR.The expression of BBC3 protein was detected by immunohistochemistry.We have analysised the correlation between the expression level of target gene BBC3 and miR-222.Results The expression level of miR-222 RNA in hepatocarcinoma tissues and adjacent tissues of liver cancer patients was significantly higher than that in normal control group and the difference was statistically significant(P<0.05).The expression level of miR-222 RNA in liver cancer tissues was higher than that in adjacent tissues,and the difference was statistically significant(P<0.05).The positive expression rates of BBC3 were 35.71%,52.86%and 80.00%in liver cancer tissues,paracancerous tissues and normal liver tissues,respectively.The expression of BBC3 were significant differences between the three groups(P<0.05).The expression levels of miR-222 and BBC3 protein was significantly negatively correlated in liver cancer tissues(γ=-0.497),and there was no statistical correlation between the adjacent tissues.Conclusion The expression of miR-222 is up-regulated in hepatocarcinoma tissues,and the expression of BBC3 is down-regulated in hepatocarcinoma tissues.There is no negative regulation between them,but no obvious regulation in adjacent tissues.The biological significance of this study needs further study.

关 键 词：MIR-222 BBC3 肝癌 相关性 

分 类 号：R735.7[医药卫生—肿瘤]