利用利用GEO数据集分析Survivin与XIAP在胃癌中的预后意义  

Analysis of prognostic significance of survivin and XIAP in gastric cancer based on gene expression omnibus(GEO) dataset

作　　者：佟笛 包博文 张斯萌[1] 杨博文[1] 刘云鹏[1] 车晓芳[1] TONG Di;BAO Bo-wen;ZHANG Si-meng;YANG Bo-wen;LIU Yun-peng;CHE Xiao-fang(Department of MedicalOncology, the First Hospital of China Medical University, Shenyang 110001, China)

机构地区：[1]中国医科大学附属第一医院肿瘤内科,辽宁省肿瘤药物与生物治疗重点实验室,辽宁沈阳110001

出　　处：《武警后勤学院学报（医学版）》2018年第9期747-751,共5页Journal of Logistics University of PAP（Medical Sciences）

基　　金：辽宁省科学技术计划项目(2015020457);2015年度留学人员科技活动项目择优资助项目(项目启动类2015-125);辽宁省中央引导地方科技发展专项资金项目(2016007010);沈阳市重点科技研发计划项目(17230901)

摘　　要：【目的】使用包含Lauren分型、分子分型的GEO数据集分析凋亡抑制蛋白X(X inhibitor of apoptosis proteins,XIAP)、survivin与胃癌患者各临床病理学参数及预后的关系。【方法】利用NCBI的GEO数据库下载基因表达谱胃癌样本数据集GSE15459,应用χ2检验分析survivin与XIAP基因表达水平与不同Lauren分型、分子分型等临床病理学参数的关系;应用Kaplan-Meier生存曲线分析上述基因的表达水平与预后总生存时间(Overall Survival,OS)的关系。【结果】(1)survivin的表达水平在不同Lauren分型(P=0.007)及分子分型(P<0.0001)之间存在差异(P=0.007),在不同性别及病理分期之间无明显差异(P>0.05)。(2)XIAP的表达水平在不同Lauren分型(P=0.041)及分子分型(P=0.002)之间存在差异,在不同性别及病理分期之间无明显差异(P>0.05)。(3)survivin与XIAP的高表达呈现出预后差的趋势,但差异无统计学意义(P值分别为0.091和0.10);两者均低表达的患者预后好于survivin和/或XIAP高表达的患者(P=0.044)。【结论】Survivin与XIAP是胃癌预后不良因素,两者联合分析更能准确判断预后。【Objective】To analyze the relationship between clinical pathological parameters and prognosis of XIAP and survivin in gastric cancer via a GEO dataset containing Lauren classification and molecular typing.【Methods】The gene expression profile of gastric cancer sample dataset GSE15459 was downloaded from the GEO database of NCBI. Chi-square test was used to analyze the relationship between the gene expression levels of survivin and XIAP and clinicopathological parameters such as different Lauren classification and molecular typing. Kaplan-Meier survival curve was used to analyze the relationship between the expression level of the above genes and overall (OS).【Results】1. The expression level of survivin was different between different Lauren types (P=0.007) and molecular typing (P<0.0001). There was no significant difference between different genders and pathological stages (P>0.05). 2. The expression level of XIAP was different between different Lauren types (P=0.041) and molecular typing (P=0.002). There was no significant difference between different genders and pathological stages (P>0.05). 3. The high expression of survivin and XIAP showed a poor prognosis, but the difference was not statistically significant (P values were 0.091 and 0.10, respectively). The prognosis in patients with low expressions of survivin and XIAP was better than that in patients with high expressions of survivin and/or XIAP (P=0.044).【Conclusion】High expressions of survivin and XIAP indicate poor prognosis of gastric cancer and the combined analysis in survivin and XIAP is more accurate in judging the prognosis.

关 键 词：胃癌 SURVIVIN 凋亡抑制蛋白X 临床病理特征 分子分型 预后 

分 类 号：R735.2[医药卫生—肿瘤]