小桐子ACO1基因的克隆与表达分析  

Cloning and expression analysis of ACO1 Gene in Jatropha curcas

作　　者：辛胡 唐利洲[2,3] 付义[1] 蒋凯文 杨蕾[1] 刘家奇[1] 马楠 田斌[1] 王海波[2,3] XIN Hu;TANG Li-zhou;FU Yi;JIANG Kai-wen;YANG Lei;LIU Jia-qi;MA Nan;TIAN Bin;WANG Hai-bo(Key Laboratory of Biodiversity Conservation in Southwest,State Forestry Administration,Southwest Forestry University,Kunming 650224,China;Center for Yunnan Plateau Biological Resources Protection and Utilization,Qujing Normal University,Qujing 655011,China;Key Laboratory of Yunnan Province Universities of the Diversity and Ecological Adaptive Evolution for Animals and Plants on YunGui Plateau,Qujing Normal University,Qujing 655011,China)

机构地区：[1]西南林业大学国家林业局西南地区生物多样性保育重点实验室,云南昆明650224 [2]曲靖师范学院/云南高原生物资源保护与利用研究中心,云南曲靖655011 [3]曲靖师范学院/云南省高校云贵高原动植物遗传多样性及生态适应性重点实验室,云南曲靖655011

出　　处：《中国油料作物学报》2019年第1期25-32,共8页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31460179)

摘　　要：为探讨ACO1基因在小桐子抗逆中的作用,本研究基于小桐子最新注释的基因组数据库,首次克隆了小桐子ACO1基因的全长CDNA序列,命名为Jc ACO1,并对其功能结构域、系统进化、基因结构及器官和低温表达特性进行了分析。结果表明,克隆的Jc ACO1基因全长为1 022bp,编码319个氨基酸,预测分子量为36. 0kDa,等电点5. 5。序列比对表明,小桐子Jc ACO1在序列中部保守性较高,具有1个Pcbc superfamily保守结构域。进化树分析显示,小桐子Jc ACO1基因与毛果杨、橡胶树、木薯的同源性较高。qRT-PCR表达分析表明,小桐子幼苗Jc ACO1基因存在器官表达特异性,在叶与茎中表达量较高,而在根中表达量较低。另外,Jc ACO1基因在三种器官中都受低温诱导,低温胁迫24h时,基因在根与茎中表达量最大。说明Jc ACO1基因表达量升高,以响应小桐子的抗冷胁迫。In order to study the role of JcACO1 gene in stress resistance of Jatropha curcas,the full-length coding frame sequence of ACO1 gene,named JcACO1(1-aminocyclopropane-1-carboxylic acid oxidase),was cloned for the first time based on the genome database of J.curcas,and its functional domain,phylogenetic evolution,gene structure and low-temperature expression characteristics were analyzed.The results showed that the length of the cloned JcACO1 gene was 1 022 bp,encoded 319 amino acids with the molecular weight of 36.04 kDa and the pI value of 5.50.Sequence alignment demonstrated that JcACO1 was highly conserved in the middle of the sequence and had a conserved domain of Pcbc superfamily.Phylogenetic tree analysis showed that the JcACO1 had high homology with Populus trichocarpa,Hevea brasiliensis and Manihot esculenta.qRT-PCR analysis revealed that Jatropha curcas seeding JcACO1 expressed in different organs,with abundant expression in leaves and stems,but scarcely in roots.In addition,JcACO1 gene was induced by hypothermia in all three organs,and the highest expression of JcACO1gene was observed in roots and stems at 24h of low temperature stress.The results showed that the expression of JcACO1 gene was increased in response to chilling stress.

关 键 词：小桐子 ACO1 基因克隆 表达分析 

分 类 号：Q75[生物学—分子生物学]