金针菇退化菌株复壮条件的优化初探  

Preliminary study on optimization of conditions for rejuvenation of degenerated strains of Flammulina filiformis

作　　者：李雪飞[1] 佟希丹 李长田[1] 付永平[1] 宋冰[1] 李玉[1] Li Xuefei;Tong Xidan;Li Changtian;Fu Yongping;Song Bing;Li Yu(Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,College of Agronomy,Jilin Agricultural University,Changchun 130118,JilinProvince,China)

机构地区：[1]吉林农业大学食药用菌教育部工程研究中心,吉林长春130118

出　　处：《食药用菌》2019年第1期52-56,共5页Edible and Medicinal Mushrooms

基　　金：长春市科技局资助项目(15SS11);公益性行业(农业)科研专项(No.201503137);吉林省教育厅项目(No.JJKH20180670KJ);高等学校学科创新引智计划资助项目(No.D17014)

摘　　要：金针菇在工厂化栽培的过程中,经常出现菌种退化问题,严重影响产量和质量。试以金针菇的退化菌株为材料,通过组织分离、菌丝尖端分离的方法进行复壮,通过接种在4种不同培养基质上进行生长速度测定,筛选最适合金针菇复壮菌株生长的基质,并考察不同光质对复壮菌株生长的影响。结果表明:构树基质可以促进金针菇复壮菌株生长;相对于白光和红光,蓝光更有利于金针菇复壮菌株的生长,并在一定程度上提高了产量。Industrial cultivation of Flammulina filiformis strains are often affected by degeneration leading to yield and quality loss.In this study,two degenerated strains of Flammulina filiformis were used as starter materials from which rejuvenated strains were obtained by tissue and mycelial tip isolation technique.The most suitable media for reviving the growth of the degenerated strains was screened by measuring their growth rate on four different media,and the effects of different light quality on their growth were also investigated.The results showed that a media prepared from Broussonetia papvrifera in addition to blue light was the best in rejuvenating the growth of degenerated strains of Flammulina filiformis and increased their yield to a certain extent.This rejuvenation method will provide technical support for commercial production of Flammulina filiformis,as well as in the preservation of strains.

关 键 词：金针菇 退化菌株 复壮 培养基质 光质 

分 类 号：S646[农业科学—蔬菜学]