TAMs/MCF-7细胞共培养体系VEGF过表达对裸鼠移植瘤Bcl-2表达的影响  被引量:1

Effect of VEGF overexpression in TAMs/MCF-7 co-culture system on the expression of Bcl-2 in transplanted tumor of nude mice

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作  者:安杰[1] 刘伟[2] 刘爽[1] 李康[2] 李志[2] AN Jie;LIU Wei;LIU Shuang;LI Kang;LI Zhi(Department of Pathology,The Bethune International Peace Hospital of PLA(Shijiazhuang 050082,China);Department of General Surgery,The Bethune International Peace Hospital of PLA(Shijiazhuang 050082,China))

机构地区:[1]中国人民解放军白求恩国际和平医院病理科,河北石家庄050082 [2]中国人民解放军白求恩国际和平医院普外一科,河北石家庄050082

出  处:《中国现代普通外科进展》2019年第1期1-4,共4页Chinese Journal of Current Advances in General Surgery

基  金:河北省卫生计生委基金资助项目(20160004)

摘  要:目的 :探讨肿瘤相关巨噬细胞(TAMs)/MCF-7细胞共培养体系中VEGF过表达对裸鼠移植瘤Bcl-2表达的影响。方法:应用PMA及IL-4体外诱导活化TAMs细胞;Transwell非接触式共培养TAMs和MCF-7细胞;MCF-7细胞分为对照组、VEGF组和共培养细胞上清组,分别建立乳腺癌裸鼠移植瘤模型,计算促瘤率;Western blot法检测移植瘤中Bcl-2表达情况。结果:VEGF组和共培养细胞上清组平均瘤重较对照组均明显增加(P<0.01),促瘤率分别为61.57%和66.61%。Bcl-2蛋白在VEGF组和共培养细胞上清组移植瘤组织中均呈高表达,且明显高于对照组(P<0.01)。结论:乳腺癌细胞可过分泌VEGF等趋化因子,招募并活化TAMs,通过VEGF/Bcl-2旁分泌环路作用,抑制肿瘤细胞的凋亡,促进肿瘤进展。Objective:To explore the effect of VEGF overexpression in TAMs/MCF-7 co-culture system on Bcl-2 expression in nude mice transplanted tumor.Method:THP-1 cell differentiation into TAMs in vitro by application of PMA and IL-4;TAMs and MCF-7 cell were co-cultured in non-contact Transwell system.MCF-7 cells were divided into control group,VEGF group and co-cultured cell supernatant group,respectively,to establish tumor transplantation model in nude mice and calculate the tumor-promoting rate;Bcl-2 expression in transplanted tumors was detected by Westernblot.Results:The mean tumor weight of VEGF group and co-cultured supernatant group was significantly increased compared with that of control group(P<0.01),and the tumor promotion rate was 61.57%and 66.61%,respectively.Bcl-2 protein was highly expressed in transplanted tumor tissues of VEGF group and co-cultured cells supernatant group,and was significantly higher than that of control group(P<0.01).Conclusion:Tumors secrete VEGF and other chemokines to recruit and activate TAMs.Inhibiting the apoptosis of tumor cells and promoting the development of the tumor by VEGF/Bcl-2 paracrine loop in tumor microenvironment.

关 键 词:乳腺肿瘤 肿瘤相关巨噬细胞 B细胞淋巴瘤/白血病-2基因 血管内皮生长因子 

分 类 号:R737.9[医药卫生—肿瘤]

 

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