米酒对兔股骨头成骨细胞破骨细胞及骨内压影响的动态观察  

Dynamic effects of rice wine on osteoblasts,osteoclasts and intraosseous pressure of rabbit femoral head

作　　者：黄思城 李宏宇[1] 席立成 韦明照 王宏润 HUANG Si-cheng;LI Hong-yu;XI Li-cheng(Department of Orthopedics,the People′s Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,China)

机构地区：[1]广西壮族自治区人民医院骨科,南宁530021 [2]柳州市工人医院骨科,广西545005

出　　处：《中国临床新医学》2019年第2期150-153,共4页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：广西自然科学基金项目(编号:2014GXNSFAA118186)

摘　　要：目的观察米酒对兔股骨头成骨细胞、破骨细胞及骨内压的影响。方法将64只兔按随机数字表法分为实验组和对照组各32只。实验组采用插胃管方式给兔灌米酒,3次/周,剂量10 ml/(kg·d)。对照组采用同样方式灌等量生理盐水,灌胃前、灌胃1、3、6个月时间点分别在实验组、对照组中随机选取兔各8只,测量兔左后肢股骨头骨内压。空气栓塞法处死兔,取右后肢股骨头作为标本,经固定、脱钙、脱水、包埋等步骤,切成5μm厚的切片,行HE染色,光镜下计算成骨细胞、破骨细胞的数量。结果灌胃前、灌胃后1个月,两组成骨细胞、破骨细胞数量及骨内压相比较,差异均无统计学意义(P> 0. 05)。灌胃后3个月、6个月,实验组成骨细胞数量较对照组少(P <0. 05),破骨细胞数量较对照组多(P <0. 05),骨内压较对照组高,差异均有统计学意义(P <0. 05)。结论长期灌服米酒(> 3个月)可使兔股骨头成骨细胞数量减少,破骨细胞数量增多,骨内压上升。Objective To observe the effects of rice wine on osteoblasts,osteoclasts and intraosseous pressure of rabbit femoral head.Methods Sixty-four rabbits were randomly divided into experimental group and control group according to random number table method,with 32 cases in each group.The experimental group was given rice wine in a dose of 10 ml/(kg·d)by inserting stomach tube,3 times a week.The control group was given the same amount of normal saline in the same way.Eight rabbits were randomly selected from the two groups respectively before and 1,3,6 months after experiment.The rabbits were sacrificed by air embolization after the internal pressure of the femoral head of left hind limbs was measured,and then the femoral head of right hind limbs was taken as specimens.The specimens underwent fixation,decalcification,dehydration,embedding and other steps,cutting to 5μm,HE staining,and the number of osteoblasts and osteoclasts were calculated under light microscope.Results There were no significant differences in the number of osteoblasts,osteoclasts and intraosseous pressure between the two groups before and one month after intragastric administration(P>0.05).The number of osteoblasts was significantly less,and the number of osteoclasts was significantly more,and the intraosseous pressure was significantly higher in the experimental group than those in the control group 3 and 6 months after intragastric administration(P<0.05).Conclusion Long-term oral administration of rice wine(more than 3 months)can decrease the number of osteoblasts,increase the number of osteoclasts and raise the intraosseous pressure in the femoral head of rabbits.

关 键 词：兔 米酒 成骨细胞 破骨细胞 骨内压 

分 类 号：R683.42[医药卫生—骨科学]