五羟色胺对大鼠舌下神经放电活性的影响  

Effects of Serotonin on Hypoglossal Nerve Discharge Activity in Rats

作　　者：汤思[1] 周秀芳[1] 胡克[1] 熊梦清 胡卫华 董明林 Tang Si;Zhou Xiufang;Hu Ke(Respiratory Department,Renmin Hospital of Wuhan University,Hubei 430060,China)

机构地区：[1]武汉大学人民医院呼吸科,430060

出　　处：《医学研究杂志》2019年第1期23-26,34,共5页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81370181;81770089)

摘　　要：目的探讨舌下神经运动核(HMN)中五羟色胺(5-HT)的浓度变化对慢性间歇低氧大鼠舌下神经放电活性的影响。方法将成年雄性Sprague-Dawley大鼠随机分为常氧组和慢性间歇低氧(CIH)组,前者分为常氧对照组和生理盐水组,后者分为CIH对照组、5-HT 1mmol/L组、5-HT 10mmol/L组、5-HT 100mmol/L组。常氧组大鼠接受常压常氧处理,CIH组给予8h/d常压间歇低氧处理,连续4周。随后给予急性间歇低氧处理,每次5min,共3次。利用脑立体定位仪在大鼠HMN微量注射不同试剂,并记录舌下神经放电活性变化。结果常氧对照组和生理盐水组大鼠舌下神经放电振幅和频率差异无统计学意义;15min、30min、45min和60min时,CIH对照组大鼠舌下神经放电振幅和频率均显著高于常氧对照组(P=0.000); 15min、30min、45min、60min时,5-HT(1mmol/L)组、5-HT(10mmol/L)组、5-HT(100mmol/L)组大鼠舌下神经放电振幅依次增大,其中,5-HT(10mmol/L)组、5-HT(100mmol/L)组大鼠舌下神经放电振幅均显著高于CIH对照组(P<0.05),而CIH对照组和5-HT(1mmol/L)组间比较,差异无统计学意义; 5-HT(100mmol/L)组大鼠舌下神经放电振幅高于5-HT(10mmol/L)组,但只在15min和30min时比较,差异有统计学意义(P<0.05); CIH各组间舌下神经放电频率比较,差异无统计学意义。结论 CIH处理可诱导大鼠舌下神经放电活性持续增强,增加HMN中5-HT浓度可增强舌下神经放电活性。Objective To investigate the effects of different concentrations of serotonin(5-HT)in hypoglossal motor nuclei(HMN)on hypoglossal discharge activity in chronic intermittent hypoxia rats.Methods Adult male Sprague-Dawley rats were randoml divided into normoxia group and chronic intermittent hypoxia(CIH)group.The former was further divided into normoxic control group and saline group,the later was then divided into CIH control group,5-HT 1mmol/L group,5-HT 10mmol/L group and 5-HT 100mmol/L group.The rats in normoxic group received normal pressures and normoxia treatment,and those in the CIH group were placed in the animal cabins of normal pressure and intermittent hypoxic condition for 4 consecutive weeks,8h/d,followed by an acute intermittent hypoxia(AIH)stimulation,a 3 times of hypoxia conduction that sustained 5min per time.Microinjection of different reagents into rat HMNs were performed using brain stereotaxic apparatus,and the changes of hypoglossal nerve discharge were recorded.Results There were no significant differences in hypoglossal nerve discharge amplitude and frequency between the normoxia control group and the saline group.At the time of 15min,30min,45min and 60min,the amplitude and frequency of hypoglossal nerve discharge in CIH control was significantly higher than the normoxia control group(P=0.000).At the time of 15min,30min,45min and 60min,hypoglossal nerve discharge amplitude in 5-HT(1mmol/L)group,5-HT(10mmol/L)group and 5-HT(100mmol/L)group successively increased.Among them,the amplitude in 5-HT(10mmol/L)and 5-HT(100mmol/L)group were significantly higher than CIH control Control(P<0.05),while there was no statistical difference between CIH control group and 5-HT(1mmol/L)group.The amplitude of hypoglossal nerve discharge in 5-HT(100mmol/L)group was significantly higher than 5-HT(10mmol/L)group only at 15min and 30min(P<0.05).There was no significant difference of hypoglossal nerve discharge frequency among CIH groups.Conclusion Conducting CIH in rats for 4 weeks can continuely increase hypoglo

关 键 词：慢性间歇低氧 放电活性 五羟色胺 舌下神经 

分 类 号：R74[医药卫生—神经病学与精神病学]