花生网斑病菌对白沙1016植株活性氧代谢和防御酶活性的影响  

Effects of Phoma arachidicola infection on active oxygen metabolism and defense enzymes changes to Baisha 1016

作　　者：刘欣宇 林英 郭永康 黄玉茜[1] LIU Xin-yu;LIN Ying;GUO Yong-kang;HUANG Yu-qian(College of Land and Environment,Shenyang Agricultural University,Shenyang 110161,China;Institute of Plant Protection,Liaoning Academy of Agricultural Sciences,Shenyang 110886,China;Fisheries College,Ocean University of China,Qingdao 266003,China)

机构地区：[1]沈阳农业大学土地与环境学院,辽宁沈阳110161 [2]辽宁省农业科学院植物保护研究所,辽宁沈阳110886 [3]中国海洋大学水产学院,山东青岛266003

出　　处：《陕西理工大学学报（自然科学版）》2019年第1期69-73,共5页Journal of Shaanxi University of Technology:Natural Science Edition

摘　　要：通过对受网斑病菌侵染的花生植株内主要防御酶活性和细胞损伤因子含量测定,探讨白沙1016抗网斑病的生理生化机制,为白沙1016品种改良及抗性品种培育提供理论依据。实验对白沙1016进行了花生网斑病抗性鉴定,并测定了感染网斑病菌的白沙1016植株在0～144 h期间O_2^-、MDA含量及4种防御酶活性的变化。结果显示:白沙1016对于花生网斑病属于感病品种,在接种网斑病菌144 h内,O_2^-和MDA含量均呈先上升后下降趋势,4种防御酶活性变化显著,POD活性变化程度最大,其次为CAT、PAL和SOD。得出结论:白沙1016的活氧代谢系统对网斑病菌的侵染有明显反应,清除病菌侵染而产生的活性氧是植株内进行最为强烈的生理生化反应,其次是对受病菌侵染的组织进行修复和防止病菌扩散的反应。To explore the physiological and biochemical mechanism of Baisha 1016 resistance to Phoma arachidicola and provide theoretical basis of improving Baisha 1016,we have identified the resistance of Baisha 1016 to Phoma arachidicola and measured the content of O2^-,the content of MDA and the changes of 4 defendant enzymes activity during 0~144 h after Baisha 1016 was infected with Phoma arachidicola.The result showed:Baisha 1016 was cultivar to Phoma arachidicola,Baisha 1016 tested bacteria inoculated leaf spot within 0~144 h,O2^-and MDA content are presented after the first drop trend,4 defendant enzymes activity changed obviously,POD activity had the most change degree,the next ones were CAT,PAL and SOD.It can thus be seen:the active oxygen metabolism system of Baisha 1016 has obvious response to Phoma arachidicola,the most strong physiological and biochemical reaction is eliminating active oxygen produced by pathogenic bacteria infection in plant,the next is the reaction that plant repairs organization infected by Phoma arachidicola and prevents Phoma arachidicola spreading.

关 键 词：白沙1016 花生网斑病 活性氧代谢 防御酶 

分 类 号：S432.1[农业科学—植物病理学]