枯草芽孢杆菌HAB-8菌株分离筛选鉴定及抑菌机理初步研究  

Isolation,Screening,Identification and Antimicrobial Mechanism of Bacillus amyloliquefaciens Strain HAB-8

作　　者：李琪敏 周婷婷 秦春秀 何其光 缪卫国 刘文波 LI Qi-min;ZHOU Ting-ting;HE Qi-guang;QIN Chun-xiu;MIAO Wei-guo;LIU Wen- bo(Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource/Institute of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,China)

机构地区：[1]海南省热带生物资源可持续利用重点实验室/海南大学热带农林学院,海南海口570228

出　　处：《黑龙江农业科学》2019年第2期28-36,共9页Heilongjiang Agricultural Sciences

基　　金：海南大学青年教师基金(hdkyxj201708);海南省自然科学基金(20163040;20153131);南繁区生物安全监测预警及控制关键技术研究与示范(201403075);海南省产学研项目(CXY20140038)

摘　　要：对枯草芽孢杆菌(Bacillus subtilis)菌株HAB-8的生物学特性及抑菌机理进行了初步研究。经形态学、生理生化特征及16SrDNA技术鉴定HAB-8菌株,应用对峙法测定了抑菌效果,对其在不同营养条件的适应性、耐盐性等进行了测定,运用光学显微镜和扫描电镜对其抑菌机理进行探究。结果表明:经形态学、生理生化特征及16srDNA技术鉴定HAB-8为B.subtilis;HAB-8菌株在6种培养基上形成的菌落,在形态上存在明显差别,直径从16.25～22.50 mm;HAB-8可在0～20%NaCl浓度的条件下生长;PDA平板对峙培养,发现HAB-8菌株,对芒果炭疽病菌等18株植物病原真菌的抑菌活性有较大差异,平均抑制率为58.52%;光学显微镜观察,抑菌带边缘的菌丝,均出现了不同程度的形态变化,菌丝扭曲变形,中部或顶端膨大变粗;孢子变形等;扫描电镜下,观察到细菌与菌丝直接接触,引起菌丝及孢子的畸形。说明拮抗细菌可能除了通过分泌活性物质来进行拮抗之外,还可能通过直接作用来抑制植物病原真菌的生长。This research was on the purpose to study the biological characteristics and antimicrobial mechanism of Bacillus subtilis strain HAB-8.First,morphology,physiological and biochemical characteristics and 16S rDNA gene sequence were used for identification of HAB-8 strains;second,the antibacterial activity was detected by flat confrontation experiments.At the same time,the adaptability of different nutritional conditions,salt tolerance was determination;Last,we used optical microscopy and scanning electron microscopy to explore its inhibitory mechanism.The results showed that the identification of bacterial strains HAB-8 was B.subtilis by morphological,physiological and biochemical characteristics,and phylogenetic analysis of 16srDNA;The colony morphology of HAB-7 is obviously different in different medium plate,colony diameter ranged from 16.25-22.50 mm;HAB-8 available in 0-20%NaCl growth conditions;HAB-8 confrontation of 18 species of pathogenic fungi,and its average bacteriostatic rate in 58.52%;there were different morphological changes in the mycelia of the treatment group,and the mycelium was distorted,the central part of mycelia was enlarged,and the spore was deformed;under scanning electron microscopy,we found the bacteria directly contact with mycelium and cause deformities of mycelium and spores.It is suggested that antagonistic bacteria may inhibit the growth of plant pathogenic fungi not only by secreting active substances,but also by direct action.

关 键 词：BACILLUSSUBTILIS HAB-8 抑菌活性 拮抗机理 扫描电镜 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]