建立杏仁核电刺激慢点燃和匹罗卡品化学点燃耐药性颞叶癫痫模型并对比癫痫发作和海马超微结构的变化  

To establish a multi-drug resistant model of temporal lobe epilepsy and to compare the characteristics of the seizure severity,amygdale EEG and hippocampal ultrastrcture

作　　者：王丽琨 周鑫 伍国锋 洪震 WANG Likun;ZHOU Xin;WU Guofeng(Department of Emergency,Affiliated Hospital of Guizhou Medical University,Guiyang 550001,China)

机构地区：[1]贵州医科大学附属医院急诊医学科,贵州贵阳550001 [2]复旦大学附属华山医院神经内科,上海200036

出　　处：《中风与神经疾病杂志》2019年第2期112-115,共4页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金地区科学基金(No.81560222)

摘　　要：目的用两种方法建立颞叶耐药癫痫模型,探讨哪种方法更适合建立多药耐药颞叶癫痫模型。方法选用SD大鼠130只,10只作为正常组,120只分别制作杏仁核和匹罗卡品模型,模型成功后用抗癫痫药苯巴比妥和苯妥英钠或卡马西平进行筛选,分别选择10只杏仁核模型和匹罗卡品模型比较两种方法建立的模型癫痫发作持续时间、发作频率、发作级别、脑电图及电镜下超微结构的变化。结果成功制作杏仁核模型31只,匹罗卡品模型29只,匹罗卡品模型癫痫发作频率(2. 09±0. 044)高于杏仁核组(1. 01±0. 037),持续时间(61. 37±4. 22)长于杏仁核组(43. 16±5. 91),而癫痫发作的级别无明显差异;经过耐药性癫痫模型的筛选,选出杏仁核耐药模型8只,匹罗卡品耐药模型12只。匹罗卡品模型与杏仁核模型相比脑电频率更高,波幅增宽,超微结构的损伤更严重。结论匹罗卡品模型自发性率高,耐药率高,脑电变化明显,超微结构损伤重,更适合耐药性癫痫机制的研究。Objective To establish a model of temporal lobe epilepsy by 2 methods,and to compare the characteristics of the amygdale EEG and seizure severity,the study investigated which one more suitable for a multi-drug resistant model of temporal lobe epilepsy.Methods A total of 130 rats,10 rats as normal group,120 rats were selected to prepare the model of epilepsy by chronic stimulation of amygaloid basal lateral nucleus or intraperitonial injection of pilocarpine.Pharmacoresistant epileptic rats were selected according their response to phenobabital and phenytoin or carbamazepine.Seizure severity,frequency,and duration,amydala EEG and the hippocampal ultrastrcture were compared between the two models.Results 31 rats were kindled successfully by amygale stimulation,the other 29 epileptic rats were prepared by intraperitonial injection of pilocarpine.We noted that the frequency of the spontaneous seizures in the pilocarpine-induced epileptic model (2.09±0.044) was higher than that of the amygdale kindled model (1.01±0.037),and the duration of epileptic seizures (61.37±4.22) was longer than that of the amygdale kindled model (43.16±5.91).Seizure severity displayed no difference between the two epileptic models.The damages of hippocampal ultrastructure in the pilocarpine-induced epileptic model was more serious than that of the amygdale kindled model.Conclusion The rate of the spontaneous epileptic seizures and the pharmacoresistance was higher in the pilocarpine-induced model of epilepsy than that of the amygdale kindling model.It was more suitable for investigating the mechanism of pharmacoresistant epilepsy.

关 键 词：耐药性癫痫 杏仁核电刺激慢点燃模型 匹罗卡品癫痫模型 

分 类 号：R742.1[医药卫生—神经病学与精神病学]