枸杞多糖对慢性低氧大鼠外周血单个核细胞中HIF-1α、VEGF mRNA和蛋白表达的影响  

Effects of Lycium Barbarum Polysaccharide on HIF-1α,VEGF mRNA and Protein Expression in Peripheral Blood Mononuclear Cells of Chronic Hypoxic Rats

作　　者：郝雪琦 关文霞 丁彦琴 张健[1] 李芳[2] HAO Xueqi;GUAN Wenxia;DING Yanqin;ZHANG Jian;LI Fang(Ningxia Medical University,Yinchuan 750004,China;Department of Respiratory, the General Hospital of Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学,银川750004 [2]宁夏医科大学总医院,银川750004

出　　处：《宁夏医科大学学报》2019年第1期14-18,23,共6页Journal of Ningxia Medical University

基　　金：国家自然科学基金(81560340);宁夏自然科学基金(NZ117158)

摘　　要：目的探讨慢性低氧对大鼠外周血单个核细胞(PBMCs)中缺氧诱导因子-1α(hypoxia inducible factors-1α,HIF-1α)、血管内皮生长因子(vascular endothelial growth factor,VEGF)表达水平及枸杞多糖(lycium barbarum polysaccharides,LBP)干预的影响。方法 28只Sprague-Dawley(SD)大鼠随机分为常氧组、低氧组、低氧/枸杞多糖组。低氧组设置为常压低氧舱(氧浓度为10%),LBP干预组分别给予LBP[200mg·(kg·d)-1]和[300mg·(kg·d)-1]2个剂量灌胃。各组干预时间均为4周。干预结束后进行血常规检测,然后收集大鼠外周血并分离PBMCs,采用荧光定量PCR和Western blot分别检测大鼠外周血单个核细胞中HIF-1α、VEGF mRNA和蛋白的表达。结果与常氧组相比,低氧组血红蛋白含量和红细胞比容升高(P均<0.01),与低氧组比较,低氧/枸杞多糖(200mg,300mg)组血红蛋白含量和红细胞比容均降低(P<0.05或<0.01);与常氧组相比,低氧组大鼠外周血单个核细胞中HIF-1α、VEGF在mRNA及蛋白表达水平均升高(P均<0.01);与低氧组相比,低氧/枸杞多糖干预的两个剂量组HIF-1α、VEGF mRNA和蛋白表达水平均下降(P<0.05或<0.01);LBP(300mg)组HIF-1α、VEGF mRNA表达低于LBP(200mg)组(P<0.05)。结论慢性低氧引起大鼠PBMCs中HIF-1α和VEGF基因表达水平上调,LBP干预能够在一定程度下调HIF-1α和VEGF基因表达。Objective To investigate the effects of chronic hypoxia on the expression of hypoxia inducible factors-1α(HIF-1α),vascular endothelial growth factor (VEGF) and Lycium Barum Polysaccharides (LBP) in peripheral blood mononuclear cells(PBMCs) of rats. Methods Total of 28 SD rats were randomly divided into normal control group, hypoxia group, hypoxia/LBP groups(200 and 300mg·(kg·d)^-1, respectively). Rats in the hypoxia and hypoxia/LBP groups were subjected to hypoxia exposure in normobaric hypoxia cabin with 10% O2 concentration. At the same time, rats in hypoxia/LBP groups were treated by intragastric administration with LBP[200mg·(kg·d)^-1] and[300mg·(kg·d)^-1], respectively. The intervention was lasted for 4 weeks. Routine blood tests were performed. The peripheral blood was collected and PBMCs was isolated from rats,and the expressions of HIF-1α and VEGF mRNA and protein were detected by fluorescence quantitative PCR and western blotting. Results Compared with the normoxic group, the hemoglobin content and erythrocyte specific volume increased in the hypoxic group(P<0.01),and decreased in the hypoxic/LBP group(200mg,300mg) compared with the hypoxic group(P<0.05 or <0.01). Compared with the normoxia group, the mRNA and protein expression levels of HIF-1α and VEGF in peripheral blood mononuclear cells of hypoxic rats were increased(P<0.01). Compared with the hypoxic group, mRNA and protein expression levels of HIF-1α and VEGF in both dose groups(200mg group,300mg group) of hypoxia/ LBP decreased(P<0.05 or <0.01). The mRNA expression of HIF -1α and VEGF in 300mg group of LBP concentration was lower than that in 200mg group of LBP concentration(P<0.05). Conclusion Chronic hypoxia induced the up-regulation of HIF-1α and VEGF gene expression in PBMCs of rats. LBP intervention could down-regulate the expression of HIF-1α and VEGF gene to a certain extent.

关 键 词：低氧 单个核细胞 缺氧诱导因子1Α 血管内皮生长因子 枸杞多糖 大鼠 

分 类 号：R364.4[医药卫生—病理学]