长链非编码RNA XLOC_009038促进食管鳞癌细胞增殖、迁移和侵袭  

Long non-coding RNA XLOC009038 promotes proliferation,migration and invasion of esophageal squamous cell carcinoma

作　　者：丁新 郑勇[1] 李静[1] 郑雪 童娟娟 杨丹平 陈卫刚[1] DING Xin;ZHENG Yong;LI Jing;ZHENG Xue;TONG Juanjuan;YANG Danping;CHEN Weigang(The First Affiliated Hospital of Shihezi University,Shihezi 832000,China)

机构地区：[1]石河子大学医学院第一附属医院,新疆石河子832000

出　　处：《实用医学杂志》2019年第3期369-374,共6页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81260362)

摘　　要：目的观察长链非编码RNA XLOC_009038对食管鳞状细胞癌EC109、EC9706细胞的增殖、凋亡、迁移及侵袭等生物学特性的影响并探讨其作用机制。方法构建XLOC_009038干扰质粒转染食管鳞癌EC109、EC9706细胞,使XLOC_009038基因表达下调。应用MTT比色法、克隆形成实验观察基因下调前后细胞增殖、克隆能力的变化;流式细胞技术检测细胞凋亡情况;Transwell测定转染前后细胞迁移及侵袭能力的变化;Western blot检测转染前后细胞内procaspase3蛋白表达量。结果两株细胞中实验组XLOC_009038基因表达明显低于对照组(P <0.001)。XLOC_009038基因表达下调后EC109、EC9706细胞的克隆、增殖能力均明显降低(P <0.05);与对照组相比实验组细胞的迁移及侵袭能力明显减弱(P <0.001);流式细胞技术显示下调XLOC_009038后EC109、EC9706两实验组细胞晚期凋亡率增高(P <0.001);Western blot显示干扰XLOC_009038后两种细胞的实验组procaspase3表达量增加(P=0.013;P <0.001)。结论 XLOC_009038与食管鳞癌的发生发展密切相关,XLOC_009038过表达可能通过抑制procaspase3来调控食管鳞癌细胞的增殖、凋亡、迁移和侵袭能力。Objective To observe the effects of long-chain non-coding RNA XLOC_009038 on the proliferation,apoptosis,migration and invasion of esophageal squamous cell carcinoma EC109 and EC9706 cells and explore its mechanism. Methods XLOC_009038 interfering plasmid was constructed and transfected into EC109 and EC9706 cells to down-regulate the expression of XLOC_009038 gene. MTT colorimetry and clonogenic assay were used to observe the changes of cell proliferation and cloning ability before and after gene down-regulation. Flow cytometry was used to detect apoptosis. Transwell was used to measure the changes of cell migration and invasion ability before and after transfection. Western blot was used to detect the expression of procaspase 3 protein in cells before and after transfection. Results The expression of XLOC_009038 gene in the two cells was significantly lower than that in the control group(P < 0.001). After down-regulation of XLOC_009038 gene expression, the cloning and proliferation ability of EC109 and EC9706 cells decreased significantly(P < 0.05). Compared with the control group,the migration and invasion ability of EC109 and EC9706 cells decreased significantly(P < 0.001). Flow cytometry showed that the apoptosis rate of EC109 and EC9706 cells increased after down-regulation of XLOC_ 009038(P < 0.001). The expression of procaspase 3 increased in the experimental group after interfering with XLOC_009038(P = 0.013;P < 0.001). Conclusions Over-expression of XLOC_009038 might be closely related to occurrence and development of the esophageal cancer. Over-expression of XLOC_009038 can enhance the proliferation,apoptosis,migration and invasion of esophageal cancer cells in vitro through the procaspase3 pathway.

关 键 词：食管鳞癌 长链非编码RNA XLOC_009038 CASPASE3 

分 类 号：R735.1[医药卫生—肿瘤]