蒙古黄芪异黄酮合成酶基因密码子偏好性分析  

Analysis of Codon Bias of IFS Gene in Astragalus. membranaceus var. mongholicus

作　　者：冯瑞云[1] 田翔[2] 程宏[3] 王慧杰[1] 梅超 左敏[1] 雷梦林[2] FENG Ruiyun;TIAN Xiang;CHENG Hong;WANG Huijie;MEI Chao;ZUO Min;LEI Menglin(Institute of Crop Sciences,Shanxi Academy of Agricultural Sciences,Key Laboratory of Crop Genetics and Molecular Improvement of Shanxi Province,Taiyuan 030031,China;Institute of Crop Germplasm Resources,Shanxi Academy of Agricultural Sciences,Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture,Taiyuan 030031,China;Institute of Maize,Shanxi Academy of Agricultural Sciences,Xinzhou 034000,China)

机构地区：[1]山西省农业科学院作物科学研究所作物遗传与分子改良山西省重点实验室,山西太原030031 [2]山西省农业科学院农作物品种资源研究所农业部黄土高原作物基因资源与种质创制重点实验室,山西太原030031 [3]山西省农业科学院玉米研究所,山西忻州034000

出　　处：《山西农业科学》2019年第3期297-303,共7页Journal of Shanxi Agricultural Sciences

基　　金：国家自然科学基金项目(31450010);山西省重点科技创新平台(201605D151002);山西省重点研发计划项目(201803D221003-3);山西省农业科学院有机旱作农业研发专项(YCX2018403)

摘　　要：异黄酮是只局限于豆科蝶形花亚科等极少数植物中的一种植物性雌激素,异黄酮合成酶(IFS)是苯丙氨酸分支代谢途径——异黄酮代谢途径的关键酶。为了解蒙古黄芪IFS基因密码子的使用特性,采用CodonW,SPSS软件及EMBOSS在线程序分析蒙古黄芪IFS基因密码子的偏好性,并分别与14个物种的IFS以及模式生物基因组进行比较。结果显示,蒙古黄芪IFS基因的密码子选择偏性较弱且较偏好以A/T结尾,而物种间IFS密码子选择偏性则存在一定差异;进化树分析表明,基于IFS编码序列聚类结果比密码子使用偏性分类结果能更准确地反映物种间的亲缘关系;密码子使用频率比较结果发现,酵母真核表达系统更适用于蒙古黄芪IFS基因异源表达系统建立,而蒙古黄芪IFS基因与模式植物基因组之间密码子使用偏性差异较小,尤其拟南芥可能为该基因转基因研究最为理想的受体。Isoflavone is a kind of plant estrogens which are restricted to only a few plants in the family Leguminosae.Isoflavone synthetase is the key enzyme of isoflavone synthesis pathway-Phyenlalanine metabolism branch pathway.To understand the codon bias of the IFS gene,one IFS gene of Astragalus.membranaceus var.mongholicus was analyzed by CodonW,SPSS software and EMBOSS online program,and compared with the 14 species IFS and the genomes of model organisms,respectively.The results showed that the selection bias of the IFS gene of Astragalus.membranaceus var.mongholicus was weaker,they were bias toward the synonymous codons with A and T at third codon position.Phylogenetic tree analysis showed that the clustering results based on IFS coding sequence could reflect the phylogenetic relationship among species more accurately than the result analyzed by RSCU(Relative Synonymous Codon Usage).It was found that the yeast eukaryotic expression system was more suitable for the establishment of the heterologous expression system of the IFS gene of Astragalus.membranaceus var.mongholicus,and the difference of codon bias between the IFS gene of Astragalus.membranaceus var.mongholicus and the genomes of the pattern plant were not significant,especially the Arabidopsis thaliana may be the most ideal receptor for the gene transfer.

关 键 词：蒙古黄芪 密码子偏好性 异黄酮合成酶 聚类分析 

分 类 号：S567.239[农业科学—中草药栽培]