汉防己碱衍生物诱导人乳腺癌MDA-MB-231细胞凋亡  

Tetrandrine Derivatives Induces the Apoptosis of Human Breast Cancer MDA-MB-231 Cells

作　　者：黄晏军 汤长宁 杨爽 潘卫东 刘杰麟[1,2] HUANG Yanjun;TANG Changning;YANG Shuang;PAN Weidong;LIU Jielin(Department of Immunology,Guizhou Medical University,Guiyang 550004,Guizhou,China;Tissue Engineering and Stem Cell Research Center,Guizhou Medical University,Guiyang 550004,Guizhou,China;The Key Laboratory of Chemistry for Natural Products of Guizhou Province,Guiyang 550004,Guizhou,China)

机构地区：[1]贵州医科大学免疫学教研室,贵州贵阳550004 [2]贵州医科大学组织工程与干细胞中心,贵州贵阳550004 [3]贵州医科大学 [4]南充市中心医院 [5]贵州省天然产物化学重点实验室,贵州贵阳550004

出　　处：《贵州医科大学学报》2019年第2期125-129,140,共6页Journal of Guizhou Medical University

基　　金：国家自然科学基金资助项目(81360349);贵州省应用基础研究计划重大专项子课题[黔科合J重大字(2015)2003];贵州省普通高等学校创新人才团队建设项目[黔教合人才团队字(2015)]

摘　　要：目的:探讨汉防己碱衍生物YZ-18对人三阴性乳腺癌MDA-MB-231细胞生长、集落形成能力以及凋亡的影响,并分析可能的药物干预机制。方法:采用不同浓度汉防己碱衍生物YZ-18干预人三阴性乳腺癌MDA-MB-231细胞后,通过MTT法检测细胞的增殖抑制率,集落形成实验检测细胞克隆形成能力,FCM法检测细胞凋亡率,蛋白印迹法检测细胞中BLM及乳腺癌易感基因1(BRCA1)蛋白表达水平。结果:不同浓度的汉防己碱衍生物YZ-18处理细胞后,细胞增殖生长明显受到抑制(P <0. 05),细胞克隆能力明显减弱(P <0. 001),细胞凋亡率明显增加(P <0. 01),BLM蛋白表达上调,BRCA1蛋白表达下调,且具有浓度依赖性。结论:汉防己碱衍生物YZ-18可抑制人三阴性乳腺癌MDA-MB-231细胞增殖,促进细胞凋亡,其作用机制可能与BLM蛋白表达上调,BRCA1蛋白表达下调有关。Objective:To investigate the effects of tetrandrine derivatives YZ-18 on proliferation,colony formation and apoptosis of human triple-negative breast cancer cell MDA-MB-231,and to analyze the possible mechanism of drug intervention.Methods:The triple-negative breast cancer MDA-MB-231 cell were treated with different concentrations of tetrandrine derivatives YZ-18,the cell proliferation was measured by MTT assay,the colony forming ability was detected by colony forming assay,flow cytometry assay was adopted to analyze the cell apoptosis rate,the cell protein expression levels of BLM and BRCA1 were detected by Western blotting assay.Results:After the triple-negative breast cancer MDA-MB-231 cell were treated with various concentration of tetrandrine derivatives YZ-18,the cell proliferation was obviously suppressed(P<0.05),cell colony forming ability was inhibited(P<0.001),the cell apoptosis rate was increased(P<0.01),and the expression levels of BLM proteins were up regulated,and that of BRCA1 was down regulated,concentration dependency was discovered.Conclusion:Tetrandrine derivatives YZ-18 can inhibit the proliferation and induce the apoptosis of human triple-negative breast cancer MDA-MB-231 cell.The mechanism may be connected with up-regulating BLM proteins and down regulating BRCA1 proteins.

关 键 词：汉防己碱 乳腺肿瘤 细胞增殖 细胞凋亡 BLM蛋白质 BRCA1蛋白质 

分 类 号：R737.9[医药卫生—肿瘤]