单次小剂量匹罗卡品对实验大鼠杏仁核电活动及海马组织的影响  

Effects of Single Low-dose Pilocarpine on Amygdala Electrical Activity and Hippocampal Tissue in Experimental Rats

作　　者：康杨婷 黄媛馨 伍国锋[2] KANG Yangting;HUANG Yuanxin;WU Guofeng(Guizhou Medical University,Guiyang 550004,Guizhou,China;Department of Emergency Neurology,Affiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou,China)

机构地区：[1]贵州医科大学 [2]贵州医科大学附院急诊神经科,贵州贵阳550004

出　　处：《贵州医科大学学报》2019年第2期153-157,共5页Journal of Guizhou Medical University

基　　金：国家自然科学基金(81760245/H0913;81560222/H0913);教育部长江学者及创新团队计划(IRT10358);贵州省优秀教育科技人才省长专项资金(1065-09);贵州省高层次人才科研特助经费(TZJF-2010-054)

摘　　要：目的:探讨单次小剂量匹罗卡品(10 mg/kg)腹腔注射对氯化锂处置大鼠杏仁核电活动及海马神经细胞数量的影响。方法:40只SD大鼠均分为正常对照组(NC组)与小剂量匹罗卡品实验组(PC组),分别在两组动物杏仁核植入记录电极,然后对PC组大鼠腹腔注射125 mg/kg的氯化锂,18 h后单次腹腔注射10 mg/kg的匹罗卡品,对照组大鼠腹腔注射125 mg/kg的生理盐水,18 h后单次腹腔注射10 mg/kg的生理盐水;于注射第7天观察两组大鼠的杏仁核电活动情况,于注射第14天观察两组大鼠海马神经元细胞的数量及GABAA受体表达的变化。结果:注射两周后,PC组大鼠没有发生点头、肢体抽搐等行为学表现,但与NC组比较,PC组大鼠杏仁核后放电的频率及波幅均明显增加,海马神经元细胞的数量及GABAA受体的表达均明显减少。结论:单次小剂量匹罗卡品能增强大鼠的杏仁核电活动并导致海马神经元的丢失,减少GABAA受体的表达,这可能是小剂量单次注射匹罗卡品致癫痫发生的机制之一。Objective:To investigate the effects of single low dose of pilocarpine(10 mg/kg)on the electrical activity of amygdala and the number of hippocampal neurons in rats injected intraperitoneally with lithium chloride.Methods:The experimental rats were divided into normal group(NC group)and low dose pilocarpine group(PC group).Recording electrodes were implanted in the amygdala of the two groups,and then the rats in the PC group were intraperitoneally injected with lithium chloride for 125 mg/kg.After 18 hours,10 mg/kg of pilocarpine was injected intraperitoneally.The NC group was injected with saline for 125 mg/kg.After 18 hours,10 mg/kg saline was injected intraperitoneally.After two weeks,the changes of amygdala electrical activity,the number of hippocampal neurons and the expression of GABA A receptor were observed.Results:In PC group,no behavior such as nodding and limb twitching was observed.However,compared with NC group,the frequency and amplitude of posterior amygdala discharges in PC group significantly increased,while the number of hippocampal neurons and the expression of GABA A receptor significantly decreased in PC group.Conclusion:single low dose pilocarpine can enhance electrical activity of amygdala in rats,and result in the loss of hippocampal neurons and decreased expression of GABA A receptor,which may be one of the mechanisms of epilepsy caused by a single low dose of pilocarpine.

关 键 词：氯化锂 癫痫 模型 动物 杏仁核后放电 GABAA受体 匹罗卡品 小剂量 大鼠 Sprague-Dawley 

分 类 号：R745.1[医药卫生—神经病学与精神病学] R338[医药卫生—临床医学]