钴铁氧体磁性固相萃取结合高效液相色谱法测定果蔬汁中4种人工合成色素  

Cobalt ferrite based magnetic solid phase extraction coupled with HPLC for the determination of four kinds of synthetic colourants in fruit and vegetable juices

作　　者：李兴红 江静 杨莉纳 李美婷 何怡凡 张红医[1] 石志红[1] LI Xinghong;JIANG Jing;YANG Lina;LI Meiting;HE Yifan;ZHANG Hongyi;SHI Zhihong(Key Laboratory of Analytical Science and Technology of Hebei Province,College ofChemistry and Environmental Science,Hebei University,Baoding 071002,China)

机构地区：[1]河北大学化学与环境科学学院河北省分析科学技术重点实验室,河北保定071002

出　　处：《河北大学学报（自然科学版）》2019年第1期41-48,共8页Journal of Hebei University(Natural Science Edition)

基　　金：教育部留学回国人员科研启动基金(47批);河北大学研究生创新资助项目(hbu2018ss21);河北大学2018年实验室开放项目(sy201801);河北大学2018年校级大学生创新创业计划训练项目

摘　　要：以钴铁氧体(CoFe_2O_4)作为磁性固相萃取(MSPE)的吸附剂,建立了MSPE-HPLC-UV检测果蔬汁中人工合成色素柠檬黄、胭脂红、日落黄、诱惑红的分析方法.采用溶剂热法制备了CoFe_2O_4,通过TEM及FT-IR对其进行表征.在酸性条件下,表面呈正电性的CoFe_2O_4与含磺酸基的合成色素主要通过静电作用相互结合,从而实现目标物的萃取.实验对材料类型和用量、溶液pH值、萃取时间等条件进行了优化.在最佳条件下,4种色素在0.04～2.0μg/mL内峰面积与质量浓度呈良好的线性关系,加标回收率在85.6%～106.6%之间,检出限为0.005～0.011μg/mL.最终将该方法用于测定果蔬汁中的合成色素,结果表明该方法快速灵敏,回收率较高,适用于实际样品中合成色素的检测.A novel magnetic solid phase extraction method based on cobalt ferrite (CoFe2O4) as adsorbent was developed for the HPLC determination of tartrazine, sunset yellow, carmine and allure red in fruit and vegetable juice. The magnetic nanoparticles were prepared via solvothermal method and characterized by transmission electron microscope (TEM) and Fourier transform infrared spectrometer (FT-IR). The analytes were adsorbed by the magnetic microspheres due to hydrophobic and electrostatic interaction under acidic condition. Several important parameters affecting the extraction efficiency, such as the type and amount of adsorbent, pH, extraction temperature and time, type and amount of desorption solvent,were investigated. Under the optimized conditions, good linearity was achieved in the range from 0.04 to 2.0 μg/mL. The limits of detection for the established method were in the range of 0.005~0.011 μg/mL. The recoveries of the four synthetic colourants ranged from 85.6% to 106.6%. The established method has been proven to be successful for the determination of the four synthetic colourants in real samples.

关 键 词：人工合成色素 钴铁氧体 磁性固相萃取 高效液相色谱法 果蔬汁 

分 类 号：O658[理学—分析化学]