基于网络药理学的补阳还五汤防治阿尔茨海默病的机制研究  

Study on the preventive mechanism of Buyang Huanwu Decoction in Alzheimer′s disease based on network pharmacology

作　　者：汪白云[1] 刘国良[2] 朴成玉 陈巧云[2] 陈景华[2] 薛慧 刘斌[2] WANG Baiyun;LIU Guoliang;PIAO Chengyu;CHEN Qiaoyun;CHEN Jinghua;XUE Hui;LIU Bin(Department of Pharmacy, Jinzhou Central Hospital, Liaoning Province, Jinzhou 121000, China;Jiamusi College of Heilongjiang University of Chinese Medicine, Heilongjiang Province, Jiamusi 154007, China;Center for Drug Safety Evaluation, Heilongjiang University of Traditional Chinese Medicine, Heilongjiang Province, Harbin 150040, China)

机构地区：[1]辽宁省锦州市中心医院药剂科,辽宁锦州121000 [2]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007 [3]黑龙江中医药大学药物安全性评价中心,黑龙江哈尔滨150040

出　　处：《中国医药导报》2019年第6期12-15,I0003,共5页China Medical Herald

基　　金：国家自然科学基金面上项目(81673621);黑龙江省自然科学基金项目(H201318);黑龙江省教育厅科学技术研究项目(12521518);黑龙江中医药大学科研基金项目(2017xy04)

摘　　要：目的预测发现补阳还五汤防治阿尔茨海默病(AD)的作用靶标,探讨多成分-多靶点-多通路的协同作用机制。方法基于网络药理学方法针对补阳还五汤的方剂组成,采用多元网络药理学方法预测补阳还五汤的体内作用靶标,通过BATMAN-TCM等数据库对比补阳还五汤抗AD药物靶标,最终采用Cytoscape软件构建补阳还五汤成分-靶点-通路的网络。结果补阳还五汤中活性成分涉及多个与AD直接相关的体内靶标,如载脂蛋白E(APOE)等。同时涉及多个代谢通路,如甘油磷脂代谢、胆碱能突触、胆固醇代谢、AD通路等。结论补阳还五汤主要通过多成分协同起效,如1-十六碳烯对载脂蛋白E调节间接起到调控疏水性淀粉样β(Aβ)沉淀形成、藏花醛对乙酰胆碱酯酶和丁酰胆碱酯酶的抑制作用等,进而延缓AD的发生发展。Objective To predict and discover the target of Buyang Huanwu Decoction in the prevention and treatment of Alzheimer′s disease (AD) and explore the synergistic mechanism of multi-component, multi-target and multi-pathway. Methods According to the composition of Buyang Huanwu Decoction, the in vivo target of Buyang Huanwu Decoction was predicted by multiple network pharmacology methods. The target of anti-AD drug was selected by BATMAN-TCM and other databases. Finally, the components of Buyang Huanwu Decoction were constructed by Cycaptose software for target path network. Results The active components of Buyang Huanwu Decoction were involved in many targets directly related to Alzheimer′s disease, such as apolipoprotein E (APOE). At the same time, it involves many metabolic pathways, such as glycerol phospholipid metabolism, cholinergic synapses, cholesterol metabolism, Alzheimer′s disease pathway and so on. Conclusion Buyang Huanwu Decoction mainly works through multi-component synergy, such as 1-hexadecence regulates apoliporotein E indirectly to regulate the formation of hydrophobic amyloid β(Aβ), the inhibition of safranal on acetylcholinesterase and butyrocholinesterase, thus delaying the occurrence and development of AD.

关 键 词：网络药理学 补阳还五汤 阿尔茨海默病 靶点 载脂蛋白E 

分 类 号：R749[医药卫生—神经病学与精神病学]