疲劳性亚健康对小鼠机械痛敏和热痛敏的影响  

Effects of fatigue-predominant subhealth on mechanical and thermal pain sensitivity in mice

作　　者：杨沅君 祁小术 张冬冬 赵永昌 殷罗悦 李倩 张咏梅 YANG Yuan-jun;QI Xiao-shu;ZHANG Dong-dong;ZHAO Yong-chang;YIN Luo-yue;LI Qian;ZHANG Yong-mei(School of Anesthesiology,Xuzhou Medical University,Xuzhou Jiangsu221004,China)

机构地区：[1]徐州医科大学麻醉学院,江苏徐州221004

出　　处：《中国药理学通报》2019年第3期387-390,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81771203;81772065);江苏省自然科学基金资助项目(No BK20161171);江苏高校品牌专业建设工程资助项目(No PPZY2015A066)

摘　　要：目的研究疲劳性亚健康对小鼠机械痛敏和热痛敏的影响。方法将昆明小鼠随机分为2组(n=10):正常对照组(control组)、疲劳性亚健康组(fatigue组)。通过观察小鼠生理行为、转棒实验,以及血常规检查,判断模型是否建立成功;通过分批次测定小鼠机械缩足反射阈值(paw withdrawal mechanical threshold, PWMT)、热缩足潜伏期(paw withdrawal thermal latency, PWTL)等行为学指标,反映机械痛敏和热痛敏的变化。结果与正常对照组小鼠相比,疲劳性亚健康组小鼠皮毛明显暗淡,转棒实验时间明显缩短(P<0.01),血常规检测无明显异常,机械痛痛阈明显升高(P<0.01),热痛痛阈明显降低(P<0.01)。结论疲劳性亚健康使小鼠的机械痛敏降低,热痛敏升高。Aim To study the effects of the fatigue-predominant subhealth on mechanical and thermal pain sensitivity in mice.Methods Kunming mice were randomly divided into two groups(n=10):normal control group(control),fatigue-predominant subhealth model group(fatigue).The physiological behavior,rota-rod test and blood routine indexes were examined.The paw withdrawal mechanical threshold(PWMT)and the paw withdrawal thermal latency(PWTL)were measured in order to reflect the change of the mechanical and thermal pain sensitivity.Results Compared to control group,the fur of model group was markedly pale,the time of rota-rod test was significantly lower(P<0.01),the data of blood cells was nearly the same,the outcome of PWMT was increased(P<0.01),and PWTL was decreased(P<0.01).Conclusions The fatigue-predominant subhealth reduces the mechanical pain sensitivity and increases the thermal pain sensitivity in mice.

关 键 词：疲劳性亚健康 机械痛敏 热痛敏 转棒实验 血常规 神经递质 

分 类 号：R-332[医药卫生] R441.1