用Micro CT评价D-半乳糖致雄性小鼠骨质疏松模型及骨化三醇的防治作用  

Evaluation of D-galactose induced osteoporosis in male mice by Micro CT and preventive effect of calcitriol

作　　者：梁美婷 吕思敏 王可欣 许碧莲 吴铁 LIANG Mei-ting;LYU Si-min;WANG Ke-xin;XU Bi-lian;WU Tie(Dept of Pharmacology,Guangdong Medical University,Zhanjiang Guangdong524023;Guangdong Medical University & Guangdong Runhe Biological Technology Co.,Ltd. Coenzyme Q10 Research Institute,Dongguan Guangdong523808,China)

机构地区：[1]广东医科大学药学院药理教研室,广东湛江524023 [2]广东医科大学.广东润和生物科技有限公司辅酶Q10联合研究中心,广东东莞523808

出　　处：《中国药理学通报》2019年第3期391-395,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81373499)

摘　　要：目的通过Micro CT扫描等方法,观察D-半乳糖致♂小鼠骨质疏松模型的股骨影像学及生物力学的变化,同时观察骨化三醇对该模型的影响。方法 17周龄SPF级♂昆明小鼠30只,随机分为正常对照组、D-半乳糖模型组、骨化三醇组,给药持续12周。实验结束时,处死小鼠,取双侧股骨做Micro CT扫描以及生物力学的三点弯曲实验。结果与正常对照组比较,模型组小鼠股骨Micro CT检测指标显示骨连接密度(Conn.D.)、骨密度(BMD)、骨体积分数(BV/TV)和骨小梁数量(Tb.N)均明显降低(P<0.05),结构模型指数(SMI)明显上升(P<0.05)。与模型组比较,骨化三醇组BMD、BV/TV、Tb.N和骨小梁厚度(Tb.Th)均明显增加(P<0.05),SMI明显降低(P<0.05)。此外,骨化三醇组的断裂载荷、弹性载荷、最大载荷和刚性系数均比模型组明显增加(P<0.05)。结论 D-半乳糖可致♂小鼠产生影像学可见的骨质疏松症,骨化三醇可明显改善D-半乳糖♂小鼠的骨小梁微观结构,并可明显增加D-半乳糖♂小鼠的股骨生物力学性能。Aim To observe the iconography and bio-mechanical changes of femur in this D-galactose induced osteoporosis model in male mice,using Micro CT and other methods,and to observe the effect of calcitriol in this model.Methods Thirty SPF male KM mice of 17 weeks old were randomly divided into control group,model group and calcitriol group,and the drug administration lasted for 12 weeks.At the end of the experiment,the mice were sacrificed and the bilateral femurs were taken for a bio-mechanical three-point bending test and a Micro CT scan.Results Compared with control group,the Micro CT index of the femur in model group showed that Conn.D.,BMD,BV/TV and Tb.N were significantly reduced(P<0.05),and SMI was raised significantly(P<0.05).Compared with model group,the BMD,BV/TV,Tb.N and Tb.Th in calcitriol group were significantly raised(P<0.05),and SMI was significantly reduced(P<0.05).What′s more,the break load,elastic load,maximum load and stiffness coefficient in calcitriol group significantly increased(P<0.05).Conclusions D-galactose can induce a visible iconography changes of osteoporosis in male mice.Calcitriol can significantly improve the micro structure of trabecular bone and increase the bio-mechanical properties of femur in D-galactose male mice.

关 键 词：骨质疏松 D-半乳糖 骨化三醇 生物力学 MICROCT 小鼠 

分 类 号：R-332[医药卫生] R322.71