通过分子标记辅助选择将耐储藏主效QTL qSS-9^(Kas)转入宁粳4号提高其种子贮藏能力  

Introducing qSS-9^(Kas) into Ningjing 4 by molecular marker-assisted selection to improve its seed storage ability

作　　者：张平 姜一梅 曹鹏辉 张福鳞 伍洪铭 蔡梦颖 刘世家[1] 田云录[1] 江玲[1] 万建民 ZHANG Ping;JIANG Yi-Mei;CAO Peng-Hui;ZHANG Fu-Lin;WU Hong-Ming;CAI Meng-Ying;LIUShi-Jia;TIAN Yun-Lu;JIANG Ling;WAN Jian-Min(State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology,Genetics and Breeding of Japonica Rice in Mid-lower Yangtze River,Ministry of Agriculture/Research Center of Jiangsu Plant Gene Engineering,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China;National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室/农业部长江中下游粳稻生物学与遗传育种重点实验室/江苏省植物基因工程技术研究中心,江苏南京210095 [2]中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程,北京100081

出　　处：《作物学报》2019年第3期335-343,共9页Acta Agronomica Sinica

基　　金：国家转基因生物新品种培育重大专项(2016ZX08001006);江苏省重点研发项目(BE2018388;BE2017368);江苏省现代作物生产协同创新中心项目资助~~

摘　　要：利用置换系SL36作为qSS-9位点上Kasalath等位基因的供体亲本,各方面农艺性状较好的宁粳4号作为轮回亲本,通过回交和自交,并使用4个与qSS-9紧密连锁的分子标记Y-10、Y-11、Y-14、Y-13进行基因型的检测筛选,对宁粳4号进行耐贮性的遗传改良,获得了既耐贮藏、大多农艺性状又接近于宁粳4号的较为稳定的优良新品系,克服了宁粳4号耐贮藏性差的弱点。获得的新品系种子在人工老化和自然老化条件下均表现出发芽率明显提高、丙二醛含量显著降低、TTC染色效果更明显,表明转入耐储藏主效QTL Qss-9^(Kas)的宁粳4号新品系耐贮性显著提高。In this paper,the chromosome segment substitution line SL36 was used as the donor parent with the Kasalath allele at the qSS-9 locus.Ningjing 4,a commercial cultivar with ideal agronomic traits,was used as a recurrent parent,through continuous self-pollination and backcrossing for four consecutive years.Four molecular markers Y-10,Y-11,Y-14,Y-13 closely linked to qSS-9 were used to screen genotypes,and molecular marker-assisted selection(MAS)was used for seed storage ability breeding for Ningjing 4.Through genetic improvement,we obtained inheritable lines with high seed storage ability.Most agronomic traits of the lines were nearly the same as those of Ningjing 4.These lines showed significantly higher germination rate,lower malondialdehyde content and more obvious TTC staining effects under artificial aging and natural aging conditions compared with Ningjing 4,indicating that the new lines with qSS-9^Kas have high seed storage ability.

关 键 词：水稻 置换系 分子标记辅助选择 耐贮性 遗传改良 

分 类 号：S511.22[农业科学—作物学]