竹叶椒对α-葡萄糖苷酶的抑制作用及机制研究  

Study on the Inhibition Against α-glycosidase and Mechanism of Zanthoxylum armature DC.

作　　者：宋彤彤 郭涛[1] 章聚宝 吴鹏法 魏黎阳[1] 谭素北 SONG Tongtong;GUO Tao;ZHANG Jubao;WU Pengfa;WEI Liyang;TAN Subei(School of Life Science and Engineering,Lanzhou University of Technology, Lanzhou 730050,China)

机构地区：[1]兰州理工大学生命科学与工程学院,甘肃兰州730050

出　　处：《食品与生物技术学报》2019年第1期58-62,共5页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(81360476)

摘　　要：采用体外抑制模型方法评价竹叶椒生物碱对α-葡萄糖苷酶(酵母菌来源、小鼠小肠来源)抑制作用,并采用Lineweaver-Burk双倒数法分析其抑制α-葡萄糖苷酶活性的机制。结果显示,竹叶椒脂溶性生物碱和水溶性生物碱都对α-葡萄糖苷酶有一定的抑制作用。竹叶椒脂溶性生物碱和水溶性生物碱对酵母来源α-葡萄糖苷酶IC50(半数抑制浓度)分别为(0.73±0.17) mg/mL、(2.74±0.28) mg/mL;脂溶性生物碱对小鼠小肠来源α-葡萄糖苷酶的IC_(50)为(1.94±0.13) mg/mL。动力学研究表明,竹叶椒生物碱提取物对α-葡萄糖苷酶的抑制作用类型为典型的非竞争性抑制。竹叶椒生物碱对α-葡萄糖苷酶有较好的抑制作用,为进一步的开发利用提供强有力的理论依据。Study on the inhibition of alkaloids from Zanthoxylum armatum DC. on α-glucosidase and mechanism. The inhibitory activity of alkaloids from Zanthoxylum armatum against α-glucosidase(from yeast and murine small intestine) were evaluated using vitro model,and the mechanism was analyzed using Lineweaver-Burk double-reciprocal analysis. Our results revealed that fat-soluble and dissoluble alkaloids both had significant inhibition on α-glucosidase,IC50 values for α-glucosidase from yeast were (0.73±0.17) mg/mL、(2.74±0.28) mg/mL,respectively. Ones for α-glucosidase from murine small intestine were (1.94±0.13) mg/mL. Moreover,the results of kinetic studies demonstrated inhibitive mechanism of alkaloids is noncompetition inhibition. The results suggested that alkaloids can effectively inhibit the α- glucosidase,and provide strong rationale for further exploitation and utilization.

关 键 词：竹叶椒 Α-糖苷酶 生物碱 非竞争性抑制 

分 类 号：TS201.4[轻工技术与工程—食品科学]