Anti-leukemic and anti-angiogenic effects of D-Limonene on K562-implanted C57BL/6 mice and the chick chorioallantoic membrane model  

作　　者：Bhavini B.Shah Ruma Baksi Kiranj K.Chaudagar Manish Nivsarkar Anita A.Mehta 

机构地区：[1]Department of Pharmacology, L.M.College of Pharmacy [2]Department of Pharmacology and Toxicology, B.V.Patel Pharmaceutical Education and Research Development Centre [3]Department of Surgery, University of Chicago

出　　处：《Animal Models and Experimental Medicine》2018年第4期328-333,共6页动物模型与实验医学（英文）

基　　金：DST-INSPIRE,Grant/Award Number:IF140131

摘　　要：Background: D-Limonene, a monoterpene from citrus fruit has been found to have chemopreventive and chemotherapeutic activities in various types of cancers. In this study, we evaluated the in vivo effect of D-Limonene on a K562-induced model of chronic myeloid leukemia(CML) in C57 BL/6 mice.Method: The tail vein injection model of K562 cells in immunocompromised C57 BL/6 mice was developed and evaluated for characteristics of the disease. The mice were treated with D-Limonene and evaluated for haematological parameters. We also evaluated the effect of D-Limonene on angiogenesis using the chick chorioallantoic membrane(CAM) assay.Results: In a complete blood count, a significant dose-dependent reduction in white blood cell, neutrophil and lymphocyte counts, but an elevation in red blood cell count and haemoglobin content was observed with D-Limonene treatment compared to the disease control or untreated group. In the CAM assay, D-Limonene produced a significant dose-dependent reduction in number of blood vessels in treatment groups compared to the vehicle-treated group.Conclusion: These studies suggest promising anti-leukemic and anti-angiogenic effects of D-Limonene in the treatment of CML.Background: D-Limonene, a monoterpene from citrus fruit has been found to have chemopreventive and chemotherapeutic activities in various types of cancers. In this study, we evaluated the in vivo effect of D-Limonene on a K562-induced model of chronic myeloid leukemia(CML) in C57 BL/6 mice.Method: The tail vein injection model of K562 cells in immunocompromised C57 BL/6 mice was developed and evaluated for characteristics of the disease. The mice were treated with D-Limonene and evaluated for haematological parameters. We also evaluated the effect of D-Limonene on angiogenesis using the chick chorioallantoic membrane(CAM) assay.Results: In a complete blood count, a significant dose-dependent reduction in white blood cell, neutrophil and lymphocyte counts, but an elevation in red blood cell count and haemoglobin content was observed with D-Limonene treatment compared to the disease control or untreated group. In the CAM assay, D-Limonene produced a significant dose-dependent reduction in number of blood vessels in treatment groups compared to the vehicle-treated group.Conclusion: These studies suggest promising anti-leukemic and anti-angiogenic effects of D-Limonene in the treatment of CML.

关 键 词：angiogenesis C57BL/6 mice CHICK chorioallantoic membrane chronic MYELOID LEUKEMIA D-LIMONENE 

分 类 号：R965[医药卫生—药理学]