Ki-67及CK13在口腔黏膜白斑上皮异常增生组织中的表达及分级病理诊断中的意义  

Expression of Ki-67 and CK13 in Oral Leukoplakia with Epithelial Dysplasia and its Significance in Pathological Diagnosis and Grading of Epithelial Dysplasia

作　　者：郑亚鸽 赵业[2] 张丽慧 姚甜 吴兰雁[5] ZHENG Ya-ge;ZHAO Ye;ZHANG Li-hui;YAO Tian;WU Lan-yan(Department of Stomatology, Chengdu Shuangliu Maternal and Child Health Hospital, Chengdu 610200, Sichuan Province;Department of Stomatology, Datong Municipal 3rd Peoples' Hospital, Datong 037008, Shanxi Province;Faculty of Stomatology, Xi'an Medical University, Xi'an 710021,Shaanxi Province;Department of Stomatology, PLA 411 Hospital, Shanghai 200081;Department of Pathology, West China Hospital of Stomatology, Sichuan University,Chengdu 610041, Sichuan Province, China)

机构地区：[1]成都市双流区妇幼保健院口腔科,四川成都610200 [2]大同市第三人民医院口腔科,山西大同37008 [3]西安医学院口腔系,陕西西安710021 [4]中国解放军第四一一医院口腔科,上海200081 [5]四川大学华西口腔医学院病理科,四川成都610041

出　　处：《口腔颌面外科杂志》2017年第5期331-337,共7页Journal of Oral and Maxillofacial Surgery

摘　　要：目的:国内外对口腔黏膜上皮异常增生的病理诊断和分级的认识存在严重分歧,其主观性较强,本研究探讨Ki-67及CK13在口腔黏膜白斑上皮异常增生组织中的联合表达,寻求口腔黏膜白斑上皮异常增生较为客观的病理辅助诊断分级指标。方法:收集口腔黏膜白斑50例,分析其临床病理特点。采用免疫组织化学S-P法检测Ki-67、CK13在伴有不同程度上皮增生的白斑中的表达情况,并对结果进行统计分析。结果:随着上皮异常增生程度的加重,Ki-67阳性细胞数增加(P<0.05);Ki-67阳性细胞在上皮内分布范围扩大,甚至波及上皮全层。CK13在OLK不伴/伴轻度上皮异常增生中高表达。大多数OLK伴中、重度上皮异常增生,CK13呈阴性表达或弱阳性表达。CK13的表达在口腔黏膜白斑伴及不伴轻度上皮异常增生组与口腔黏膜白斑伴中、重度上皮异常增生组之间,差异有统计学意义(P<0.05)。结论:Ki-67、CK13的联合应用有助于口腔黏膜白斑上皮异常增生程度的判断,为病理诊断提供了较为客观的依据。Objective: To study the expression of Ki-67 and CK13 in oral leukoplakia(OLK) and to assess its significance in diagnosis of oral dysplasia. Methods: A total of 50 cases of oral leukoplakia were retrieved from department of oral pathology, stomatology hospital of Sichuan University. The immunohistochemical staining of Ki-67 protein and CK13 protein were used in above cases. Results: The number of positive expression of Ki-67 labeling cells presented in a positive correlation with the differentiation degree of the epithelial dysplasia from mild to severe (P<0.05). Ki-67-immunopositive cells were spread throughout wider areas even in the whole epithelial layer in poor dysplasia cases. CK13 was strongly expressed in OLK without/with mild dysplasia. In most cases, the expression of CK13 in moderate and severe dysplasia was negative or weak. The expression of CK13 between OLK without/mild dysplasia and OLK with moderate/ severe dysplasia showed a significant difference statistically (P<0.05). Conclusion: Ki-67 and CK13 are useful markers for diagnosis and grading OLK with epithelial dysplasia.

关 键 词：口腔黏膜白斑 上皮异常增生分级 KI-67 CK13 

分 类 号：R781.5[医药卫生—口腔医学]